The aim. aim of the work was to study indicators spermatogenesis in lake frog, Pelophylax ridibundus (Pallas, 1771), zone influence emissions from Burshtyn Thermal Power Plant (TPP).
 Materials and methods. Urinal sperm testes samples were taken male frogs under conditions anthropogenic pollution (Burshtyn industrial area). Such parameters as concentration ejaculate, number live spermatozo...

Introduction: No definitive treatment is available for Lupus. Moringaoleiferais one of promisingnovel treatments in Lupus trough anti inflammation and immunosupresion. Mean platelet volume andneutrophil to lymphocyte ratio used measure degree lupus activities.
 Objective: Our study wasaimed identify the effect Moringaoleiferaextract on neutrophil lupus.
 Methods: This experimental was...

Large numbers of vacuoles (10(6)-10(7)) have been isolated from Sorghum bicolor protoplasts and analyzed for the cyanogenic glucoside dhurrin. Leaves from light-grown seedlings were incubated for 4 hours in 1.5% cellulysin and 0.5% macerase to yield mesophyll protoplasts which then were recovered by centrifugation, quantitated by a hemocytometer, and assayed for cyanogenic glucosides. Mature va...

The aim of this study was to develop a method for assessing the growth photoautotrophs on plaster coatings, which will be used reliably assess resistance these materials photoautotrophic in simulation long-term exposure. In course study, mineral and silicone substrates were inoculated with mixture Stichococcus bacillaris, Nostoc commune, Pseudochlorella signiensis, Coenochloris incubated 28 day...

Introduction Hemocytes are an essential component of the invertebrate innate immune system (Strand 2008). Their close similarities to vertebrate white blood cells enable their use as a tractable model for immunological research. The quantification of hemocytes in the hemolymph (invertebrate blood) is one way of assessing the efficacy of the immune response because the absolute number of hemocyt...

The aim of this paper is to compare three different methods for counting white blood cells [WBC] (Natt and Herrick method, estimation with 1,000 and 2,000 erythrocytes) and three methods for counting total thrombocytes [TT] (Wojtaszek method, estimation with 1,000 and 2,000 erythrocytes) in a South American freshwater turtle species, Podocnemis expansa, Schweigger 1812 (Reptilia, Pelomedusidae)...

The number of salivary gland malaria sporozoites (sporozoite load) was determined by hemacytometer counts for 2,055 field-collected Anopheles mosquitoes from Kilifi District, Kenya. Of 48 gland-positive Anopheles gambiae s.l., sporozoite loads ranged from 125 to 79,875, with a geometric mean of 1,743 sporozoites per infected mosquito. About half of the infected mosquitoes had sporozoite loads <...

OBJECTIVE The purpose of this study was to use a Collagenase-Dispase assay to investigate the potential of a new storage media, coconut water, in maintaining viable periodontal ligament (PDL) cells on simulated avulsed teeth. STUDY DESIGN Fifty freshly extracted human teeth were divided into 3 experimental groups and 2 control groups. The positive and negative controls corresponded to 0 minut...

The S-FCM uses flow cytometry technology to measure sperm concentrations. Semen samples from 104 men attending a male infertility clinic were used to evaluate the reproducibility of results and the carryover rate with the S-FCM by performing between- and within-run imprecision analyses. In addition, sperm concentrations measured with the S-FCM were compared with those obtained by manual analyse...

or cerebrospinal fluids have been misidentified as leukocytes by the Coulter method.6,7 Once injected intrapleurally, some of the talc particles will remain in the pleural space for a variable length of time. These residual particles will frequently be mixed with the induced pleural fluid. Investigators should beware that in experimental settings involving the use of talc, total cell counts in ...