Reticulocytes from newborn infants with Rh isoimmune hemolytic disease actively incorporated radioactive amino acids in vitro into hemoglobins F and A. Approximately 50% of the reticulocytes appeared capable of synthesis of both of these hemoglobins within the same cell, as demonstrated by the selective elution technique of Betke and Kleihauer. An isoleucine analogue, L-O-methylthreonine, inhib...

Tryptic peptides were isolated from the ,8 chains of sheep hemoglobins A, B, and C and placed in linear array through the use of nonuniform radioactive label. Nearly complete sequence analysis indicates that the /3 chains of hemoglobins A and B contain 145 amino acids while the p chain of hemoglobin C lacks 4 residues in its NH&erminal portion and contains only 141 amino acids. The sum of diffe...

Using 12CO and 13CO liganded protein and 406 nm laser excitation, multiple stretching (upsilon(Fe-CO), upsilon(C-O)) and bending (delta(Fe-C-O)) modes have been identified in the resonance Raman spectra of monomeric and polymeric Glycera hemoglobins. While the monomer fraction Glycera dibranchiata hemoglobin has upsilon(Fe-CO) = 496 cm-1, two distinct upsilon(Fe-CO) modes are found at 498 cm-1 ...

Hemoglobin is an important protein found in the red cells of many animals. In humans, the hemoglobin is mainly distributed in the red blood cell. Single amino acid substitution is the main pathogenesis of most hemoglobin disorders. Here, the author used a new gene ontology technology to predict the molecular function and biological process of four important hemoglobin disorders with single subs...

Hemoglobins (Hb) Yakima and Kempsey were purified from patients' blood with diethylaminoethyl cellulose column chromatography. The oxygen equilibrium curves of the two hemoglobins and the effects of organic phosphates on the function were investigated. In 0.1 M phosphate buffer, Hill's constants n for Hb Yakima and Hb Kempsey were 1.0 to 1.1 at the pH range for 6.5 to 8.0 and the oxygen affinit...

Hemoglobins from three sharks, the porbeagle (Lamno nusus), the dusky (Carcharhinus obscurus), and the mako (Zsurus oxyrinchus), and four bony fishes, the big-eye tuna (Thunnus obesus), the swordfish (Xiphias gladius), the carp (Cyprius carpio), and the smallmouth bass (Micropefrus dolomieu), have been examined by a variety of techniques to determine both their molecular structure and their kin...

\TO CLASSES of amino acid substitutions in hemoglobins have profound effects on the major function of this protein, oxygen transport. The first includes the hemoglobins M’ and hemoglobin Kansas,2 which result in cyanoSis in heterozygous subjects and no significant erythrocytosis. The second class, represented by hemoglobin Chesapeake3 and hemoglobin Yakima,4 is assocated with erythrocytosis in ...

We cloned two hemoglobin genes from Arabidopsis thaliana. One gene, AHB1, is related in sequence to the family of nonsymbiotic hemoglobin genes previously identified in a number of plant species (class 1). The second hemoglobin gene, AHB2, represents a class of nonsymbiotic hemoglobin (class 2) related in sequence to the symbiotic hemoglobin genes of legumes and Casuarina. The properties of the...

Hb J-Iran [beta77(EF1)His-Asp] is a rare hemoglobin variant, described first in Iran by Rahbar et al. in 1967 . To date, several abnormal hemoglobins have been described from different regions of Turkey . In Turkey, the first Hb J-Iran [beta77(EF1) His-Asp] case was reported by Arcasoy et al. . There are four reported cases from the Turkish population, mostly from our group. These reported case...

There is now a great deal of physical and chemical evidence that horse and human hemoglobins undergo a change of protein conformation on oxygenation.' 9 The molecular mechanism whereby these hemoglobins show cooperative or sigmoidal oxygen binding has remained obscure, however. In order to understand this "heme-heme" interaction, one must obtain structural information on partially oxygenated he...