Fully human antibodies have minimal immunogenicity and safety profiles. At present, most potential antibody drugs in clinical trials are humanized or fully human. Human mostly generated using the phage display method (in vitro) by transgenic mice vivo); other methods include B lymphocyte immortalization, human–human hybridoma, single-cell polymerase chain reaction. Here, we describe a structure...

Glycosylation patterns in antibodies critically determine biological and physical properties but their precise control is a significant challenge in biology and biotechnology. We describe herein the optimization of an endoglycosidase-catalyzed glycosylation of the best-selling biotherapeutic Herceptin, an anti-HER2 antibody. Precise MS analysis of the intact four-chain Ab heteromultimer reveals...

We report on two cases of women on trastuzumab therapy for breast cancer who became pregnant and delivered healthy live infants. At the time of reporting the children are growing and developing normally (ages 3 and 2).

Research Highlights and Abstract This article: Covers new empirical terrain in the study of depoliticisation, with an in-depth case study of health technology regulation;Analyses depoliticisation from a novel analytical perspective, examining how depoliticised institutions are resilient to external pressure for politicisation;Posits a distinctive framework for analysing resilience, drawing on c...

Abstract Bio-responsive nanocomposites with facile fabrication and rational design are of great importance in the diagnosis treatment cancer. Herein, combination bioimaging due to presence QD controlled drug delivery via nanogel was employed for cancer treatment. To this aim, we fabricated a traceable bioresponsive fluorescent active composite by integrating creatinine-functionalized carbon dot...

Background Anthracyclines and Herceptin are well known amongst chemotherapeutic agents to cause cardiotoxicity. Anthracyclines, particularly in combination with, Herceptin are seen in studies to cause cumulative, dose-related, progressive myocardial damage with decrease in left ventricular(LV) ejection fraction (EF) ultimately leading to clinical heart failure, which can be irreversible. There ...

We demonstrate a facile, one-step, protein-directed approach to preparing a new functional reduced graphene oxide via simultaneous reduction and surface functionalization of graphene oxide sheets with herceptin and show its potential application as a novel biological imaging agent.