purpose : the evaluation of the presence of herpes simplex virus (hsv) in corneal scars by real-time polymerase chain reaction (pcr) and comparison of the results with histopathologic findings and clinical diagnoses methods : eighty-seven corneal scar samples obtained after penetrating keratoplasty (pk), were selected after reviewing the records from 2006 to 2010. nucleic acid was extracted fro...

background herpes simplex virus infections are very common worldwide. the virus can cause infection in various body parts, especially eyes and the nervous system. therefore, an early diagnosis and highly sensitive method is very helpful. objectives the present study sought to investigate the efficiency of real-time taqman probe pcr in the diagnosis of hsv infection in suspected patients. patien...

The sensitization of herpes simplex virus by immunoglobulin G (IgG) antibodies was studied. Although herpes simplex virus sensitized by IgG could not be neutralized by IgA antibodies known to neutralize a similar number of unsensitized viruses, this protection could be overcome by larger amounts of IgA antibodies. This suggests that the effective neutralization of herpes simplex virus in ocular...

An avidin-biotin complex system was compared with three commercially available immunofluorescence kits for serotyping herpes simplex virus isolates from clinical specimens. Sensitivity values showed that the Electro-Nucleonics and Immulok reagents were useful in detecting the presence of virus, whereas the predictive values showed that the Syva and Immulok reagents possessed adequate discrimina...

Normal bone marrow cells from a donor positive for herpes simplex virus were transformed with Epstein-Barr virus. The resulting lymphoblastoid cell line has secreted immunoglobulin G1 of the kappa type continuously for 2 years. This immunoglobulin, detected both on the cell surface and in the cytoplasm, reacts with cells infected with herpes simplex virus. It defines an antigen that comigrates ...

A rat cell line, RE1, oncogenically transformed by a herpes simplex virus type 2 ts mutant (ts 1), has been demonstrated to contain herpes simplex virus type 2-specific thymidine kinase activity, as have two of four tumors induced in rats by inoculation of these transformed cells. A high proportion of sera from tumor-bearing rats (5 of 11) have detectable antibody against herpes simplex virus t...

BACKGROUND Herpesvirus infections often complicate the clinical course of patients with inflammatory bowel disease; however, invasive disease due to herpes simplex virus is distinctly uncommon. METHODS We present a case of herpes simplex virus colitis and hepatitis, review all the previously published cases of herpes simplex virus colitis, and discuss common clinical features and outcomes. We...

OBJECTIVE The purpose of this study was to determine the cost-effectiveness of routine antenatal screening for herpes simplex virus 1 and 2 in women without a known history of genital herpes. STUDY DESIGN Decision analysis was used to compare 3 treatment strategies to prevent neonatal herpes infection in women without a known history of genital herpes simplex virus: (1) the current standard o...

objective: there are a number of published data indicating in vitro anti-hsv activity of some of iranian herbal extracts with no systematic review to discuss these results. therefore, this article was aimed to review and discuss the methods carried out and the phytochemistry and bioactivity of the extracts used and also conclusions provided in these publications. materials and methods: publishe...

background and objectives: essential oils are complex mixtures containing compounds of several different functional- group classes. depending on the structure, we can distinguish monoterpenes, phenylpropanes, and other components. here in this study two monoterpene compounds of essential oils, i.e. β-pinene and limonene were examined for their antiviral activity against herpes simplex virus typ...