Using 16S rDNA gene sequencing technique, three different species of non-symbiotic bacteria of entomopatho-genic nematodes (EPNs) (Steinernema sp. and Heterorhabditis sp.) were isolated and identified from infected insect cadavers {Galleria mellonella larvae) after 48-hour post infections. Sequence similarity analysis revealed that the strains SRK3, SRK4 and SRK5 belong to Ochrobactrum cytisi, ...

The citrus mealybug, Planococcus citri (Risso, 1813) (Hemiptera: Pseudococcidae) is one of the major pests Citrus orchards in Turkey. Management P. quite challenging due to its cryptic and polyphagous feeding behavior. In current study, control potential native entomopathogenic nematodes (EPNs) species (Heterorhabditis indica 216-H, H. bacteriophora FLH-4H, Steinernema carpocapsae E-76, S. felt...

We determined virulence of seven Heterorhabditis bacteriophora strain GPS11 inbred lines possessing superior infective juvenile longevity, and heat and ultra violet radiation tolerance against white grubs Popillia japonica and Cyclocephala borealis. At 1 and 2 weeks after treatment, inbred line A2 was significantly more virulent towards P. japonica compared to the parent strain GPS11 and inbred...

The potential for entomopathogenic nematodes to control ßies in cattle feedlots was determined by screening 40 strains representing 8 species of Heterorhabditis Poinar and 5 species of Steinernema Travassos for virulence toward 3rd-instar house ßies (maggots), Musca domestica L. None of the 22 strains of Heterorhabditis infecting maggots caused signiÞcant levels of mortality in a Þlter paper as...

Entomopathogenic nematodes are generally considered beneficial organisms. However, they can affect beneficial insects such as parasitoids. The interaction between the entomopathogenic nematodes Heterorhabditis bacteriophora Poinar (Rhabditida: Heterorhabditidae) and Steinernema carpocapsae Weiser, and the parasitoid Microplitis rufiventris Kokujev (Hymenoptera: Braconidae) was investigated in t...

différences! [editorial] [editorial]onomic way of identifying and assigning nematodes to taxons, which had already been determined either by comparative sequence analysis of nuclear rDNA internal transcribed spacer (ITS) region or by other methods of molecular or conventional taxonomy, is provided. Molecular identification of entomopathogenic nematodes (EPN) can be upgraded by basing it on Phas...

Entomopathogenic nematodes in the genus Steinernema and Heterorhabditis have been successfully commercialized as biological control agents for a variety of curculionid species. Nematode-based products have been introduced for: 1) root weevils, Otiorhynchus spp. 2) billbugs, Sphenophorus spp. 3) the apopka weevil, Diaprepes abbreviatus, and 4) the citrus root weevil, Pachnaeus litus. Several oth...

Five bacterial strains were isolated from the hemocoel of the greater wax moth larvae (Galleria mellonella) infected with the entomopathogenic nematodes: Heterorhabditis bacteriophora HP88, Heterorhabditis indicus RM1 and Heterorhabditis sp (S1), Steinernema abbasi and Steinernema sp. (S II). Strains were identified as Photorhabdus luminescens HRM1, P. luminescens HS1, P. luminescens HP88, Xeno...

The study investigates the effect of temperature on the infectivity of two entomopathogenic nematodes, viz. Steinernema sp. and Heterorhabditis indica, locally isolated from Mizoram, northeastern India, using last instar larvae of greater wax moth, Galleria mellonella, as an insect host. Two hundred infective juveniles/larva of wax moth were exposed to different temperatures, viz. 10, 15, 20, 2...