Corynebacterium glutamicum mutant KY9707 was originally isolated for lysozyme-sensitivity, and showed temperature-sensitive growth. Two DNA fragments from a wild-type C. glutamicum chromosomal library suppressed the temperature-sensitivity of KY9707. These clones also rescued the lysozyme-sensitivity of KY9707, although partially. One of them encodes a protein of 382 amino acid residues, the N-...

Ion channelopathies have now been described in many well-characterized cell types including neurons, myocytes, epithelial cells, and endocrine cells. However, in only a few cases has the relationship between altered ion channel function, cell biology, and clinical disease been defined. Hyperinsulinism in infancy (HI) is a rare, potentially lethal condition of the newborn and early childhood. Th...

Inflammation and neuroprotective strategies in the immature brain after hypoxic-ischemic brain injury Pernilla Svedin, 2008, Perinatal Center, Department of Physiology, Institute of Neuroscience and Physiology, University of Gothenburg, Box 432, 405 30 Göteborg Sweden Perinatal brain injury, as a result of hypoxia-ischemia (HI) or infection/HI, is a major cause of acute mortality and neurologic...

Objectives Mutations in mitochondrial DNA cause a variety of clinical phenotypes ranging from a mild hearing impairment (HI) to severe encephalomyopathy. The MT-TS1 gene is a hotspot for mutations causing HI. The m.7510T>C mutation in MT-TS1 has been previously associated with non-syndromic HI in four families from different ethnic backgrounds. Materials and Methods We describe the clinical, ...

The nucleoprotein (NP) gene of type B influenza virus was detected by reverse transcription polymerase chain reaction (RT-PCR) from the cerebrospinal fluid (CSF) of a patient presenting with ataxia due to cerebellitis. The CSFwas obtained 7 and 9 weeks after flu syndrome occurred, suggesting persistence of viral genes in the central nervous system (CNS). Although an unusually high serum hemaggl...

The folding and three-dimensional (3D) organization of chromatin in the nucleus critically impacts genome function. The past decade has witnessed rapid advances in genomic tools for delineating 3D genome architecture. Among them, chromosome conformation capture (3C)-based methods such as Hi-C are the most widely used techniques for mapping chromatin interactions. However, traditional Hi-C proto...

The objective of this research was to test whether sodium hydroxybutyrate (GHB-Na) protects rat neonatal brain against hypoxia-ischemia (HI). Specifically, the objective was to determine the effect of GHB-Na administration on the expression of N-methyl-D-aspartate subunit (NR2B) mRNA in the rat hippocampus. Seven-day-old Sprague-Dawley rats were subjected to ligation of the left carotid artery ...

Most cases of hyperinsulinism of infancy (HI) are caused by mutations in either the sulfonylurea receptor-1 (SUR1) or the inward rectifying K channel Kir6.2, two subunits of the -cell ATP-sensitive K channel (KATP channel). Histologically, HI can be divided into two major subtypes. The diffuse form is recessively inherited and involves all -cells within the pancreas. Focal HI consists of adenom...

We have cloned the gene encoding RNase HII (RNase HIIPk) from the hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1 by screening of a library for clones that suppressed the temperature-sensitive growth phenotype of an rnh mutant strain of Escherichia coli. This gene was expressed in an rnh mutant strain of E. coli, the recombinant enzyme was purified, and its biochemical properties were ...