My husband, Carl B. Camras, MD (chairman of the Department of Ophthalmology and Visual Sciences, University of Nebraska Medical Center, Omaha), died at age 55 years in 2009. His dying wish was to be remembered for being the first to hypothesize that prostaglandins lower intraocular pressure and had potential as a medication to treat glaucoma. I reviewed the research he performed as an undergrad...

From trauma to amnesia to senior moments, memory has been a major plot line in films since the 1942 classic, Random Harvest. John Seamon, an author and professor of psychology whose research includes how a camera aids memory and the impact of storytelling on memory, has shifted his lens to focus on how memory has been portrayed in one of the world's most beloved art forms.

This study aims to describe the racial discrimination from white people against black that was formerly a slave by analyzing Langston Hughes' poems; I, Too, To Black Beloved, The White Ones, and My Beloved. Presentation of can be seen act prejudice, insulting, words used, suppression people. These poems represent poet's feeling social phenomena happened. data were analyzed utilizing new histori...

Mamin-Sibiryak belonged to several cultural and educational public organizations, was familiar with many representatives of the provincial intelligentsia, who became prototypes for a cycle his essays called “Beloved People” (1896). The are devoted Ural local historians, most often nonprofessional scientists, collected bit by information about history culture Urals: P. M. Vologodsky, N. K. Chupi...

The polar flagellum of Vibrio alginolyticus is rotated by the sodium motor. The stator unit of the sodium motor consists of four different proteins: PomA, PomB, MotX and MotY. MotX and MotY, which are unique components of the sodium motor, form the T-ring structure attached to the LP ring in the periplasmic space. MotY has a putative peptidoglycan-binding motif in its C-terminal region and MotX...

The recombinant expression of soluble proteins in Escherichia coli continues to be a major bottleneck in structural genomics. The establishment of reliable protocols for the performance of small-scale expression and solubility testing is an essential component of structural genomic pipelines. The SSGCID Protein Production Group at the University of Washington (UW-PPG) has developed a high-throu...

BACKGROUND Functional Genomics, the systematic characterisation of the functions of an organism's genes, includes the study of the gene products, the proteins. Such studies require methods to express and purify these proteins in a parallel, time and cost effective manner. RESULTS We developed a method for parallel expression and purification of recombinant proteins with a hexahistidine tag (H...

A practically simple top-down process for the exfoliation of graphene (GN) and few-layer graphene (FLG) from graphite is described. We have discovered that a biocompatible amphiphilic pyrene-based hexahistidine peptide is able to exfoliate, functionalize, and dissolve few layer graphene flakes in pure water under exceptionally mild, sustainable and virtually innocuous low intensity cavitation c...

Many proteins that accumulate in the form of insoluble aggregates when they are overproduced in Escherichia coli can be rendered soluble by fusing them to E. coli maltose binding protein (MBP), and this will often enable them to fold in to their biologically active conformations. Yet, although it is an excellent solubility enhancer, MBP is not a particularly good affinity tag for protein purifi...