A practically simple top-down process for the exfoliation of graphene (GN) and few-layer graphene (FLG) from graphite is described. We have discovered that a biocompatible amphiphilic pyrene-based hexahistidine peptide is able to exfoliate, functionalize, and dissolve few layer graphene flakes in pure water under exceptionally mild, sustainable and virtually innocuous low intensity cavitation c...

Many proteins that accumulate in the form of insoluble aggregates when they are overproduced in Escherichia coli can be rendered soluble by fusing them to E. coli maltose binding protein (MBP), and this will often enable them to fold in to their biologically active conformations. Yet, although it is an excellent solubility enhancer, MBP is not a particularly good affinity tag for protein purifi...

Since the 1860s, biologists have repeatedly referred to Lamarck as one of the founding fathers of modern evolutionary doctrines, a claim hotly denied by most of their colleagues. Lamarck’s name has often been evoked to contrast his insistence on acquired individual variations with the mainstream interpretation of the Darwinian doctrine, which stresses the constant presence within a population o...

a practical acquaintance with this subject could gain an; assistance from figures of so sketchy a nature. The treating of eclampsia is a subject which the author has made peculiar > his own: his practice is to deliver immediately, or as soona possible if eclampsia has already set in, and to do this un deep anaesthesia. In cases of post-partum hemorrhage W* atony the author recommends the pluggi...

A surface plasmon resonance imaging-based Ni(2+)-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.

Fluorescent probes are essential for the exploration of protein function, detection of molecular interactions, and conformational changes. The nitrilotriacetic acid derivatives of different chromophores were successfully used for site-selective noncovalent fluorescence labeling of histidine-tagged proteins. All of them, however, suffer from the same drawback--loss of the fluorescence upon bindi...

Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes. Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, S...

Abstract Optimal and efficient immunization of large networks remains a challenging task. Many theories approaches have been suggested, however most them require complete knowledge the underlying network structure. Here, we study targeted strategy that incorporates fact there is often limited on Previous work has suggested ‘acquaintance’ immunization, where rather than selecting random individu...

Ranking, m.d., m r.c.s., i.m.s. Thacker, Spink & Co., Calcutta. This brochure contains much sound advice for the young subaltern, civilian, or merchant's assistant as to how to take care of himself during the first }rears after his arrival in India, which is set forth in simple terms and in a very readable form by an observer with a long experience of India and an extensive acquaintance of the ...