A big challenge for our century concerns discovering new foods, including re-discovering ancient wheat. Phenolic and steryl ferulates composition of Tuscan Campania wheat samples was evaluated by HPLC-DAD-MS/MS. Cinnamic acids flavonoids were expressed as ferulic acid schaftoside equivalents, respectively. Agronomic conditions with a higher rate plants/m2 promoted amounts phenols in most the va...

weichang’an (wca) pill, a traditional chinese patent medicine consisting of ten chinese medicinal herbs, has been used to treat irritable bowel syndrome and functional dyspepsia for several decades. in this study, twelve bioactive constituents in the methanol extract of wca were accurately identified since ms/ms fragmentation behavior of the references and the standards by using hplc-dad-esi-ms...

Abstract In this study, the optimization and performance evaluation of a high-performance liquid chromatography with diode array detection (HPLC–DAD) method for simultaneous determination four sweeteners (acesulfame-potassium, saccharin, aspartame, rebaudioside A), two preservatives (sodium benzoate potassium sorbate), caffeine in sugar-free drinks are presented. The separation was carried out ...

Carotenoids are biologically active phytochemicals present as micro-components in fruits and vegetables, being responsible for their yellow, orange and red colors. The chromatographic behavior and the UV absorption spectrum provided by HPLC-DAD analysis constitute the clues for their identification. Mushrooms are of increasing importance in modern nutrition and medicine, due to the presence of ...

In this paper high-performance liquid chromatography with diode array UV detection (HPLC/DAD) after SPE sample pretreatment for simultaneous analysis of pharmaceuticals from three different classes: enrofloxacin, oxytetracycline and trimethoprim, has been developed, optimised and validated. The chromatographic separation was developed on spiked wellspring water samples and checked on model wast...

Chemical analysis of fermentation products from two Australian Streptomyces isolates yielded all four known and twelve new examples of the rare reveromycin class of polyketide spiroketals, including hemi-succinates, hemi-fumarates and hemi-furanoates. Reveromycins were identified with the aid of HPLC-DAD-MS and HPLC-DAD-SPE-NMR methodology, and structures were assigned by detailed spectroscopic...

High-performance liquid chromatography-diode array detector (HPLC-DAD) coupled with electronspray mass spectrometry (ESI-MS) was used to detect the flavonoid profile in lemon juices obtained from the main Sicilian cultivars (Femminello comune, Monachello, and Interdonato). Significant amounts of an unusual constituent were found in the lemon juice of the above-mentioned cultivars together with ...

Amaç: Bu çalışmanın amacı, farklı nesil florokinolonlardan; siprofloksasin, levofloksasin, enrofloksasin ve moksifloksasinin ayrılması eş zamanlı analizine olanak sağlayan yeni, kolay, hızlı hassas bir HPLC-DAD yöntemi geliştirmektir. Gereç Yöntem: Literatürlerde, etken maddede florokinolonların tek başına veya ikili karışımlarının ayrılmaları, analizi miktar tayinleri ile ilgili çeşitli yöntem...

Xanthohumol (1), isolated from hop, was fed to rats in a dose of 1000 mg kg(-1) body weight. The faeces of the animals were collected after 24 and 48 h and analysed for metabolites of 1. Approximately 89% of the recovered flavonoid-compounds consisted of unchanged 1. Sixteen metabolites and six previously known metabolites were isolated and characterized by coupling techniques (HPLC-NMR, HPLC-M...