BACKGROUND AND OBJECTIVES Human Papillomavirus (HPV) infection is a major risk factor for adenocarcinoma of the cervix. The high-risk types of the virus such as HPV16 and HPV18, which possess the E6 and E7 oncogenes, are responsible for approximately 50% of all cervical cancers. A rapid, sensitive and specific test has been proposed for detection of HPV to improve cervical cancer screening prog...

BACKGROUND Prophylactic vaccines are available for women and girls not yet infected with HPV, but women already infected with HPV need a treatment to prevent progression to high-grade cervical lesions and cancer. GTL001 is a bivalent therapeutic vaccine for eradicating HPV-infected cells that contains HPV16 E7 and HPV18 E7 both fused to detoxified adenylate cyclase from Bordetella pertussis, wh...

UNLABELLED Most DNA viruses associate with, and reorganize, nuclear domain 10 (ND10) bodies upon entry into the host nucleus. In this study, we examine the roles of the ND10 components PML, Sp100, and Daxx in the establishment of human papillomavirus type 18 (HPV18) infection of primary human keratinocytes. HPV18 DNA or HPV18 quasivirus was introduced into primary human keratinocytes depleted o...

UNLABELLED Transcripts of human papillomavirus 16 (HPV16) E6 and E7 oncogenes undergo alternative RNA splicing to produce multiple splice isoforms. However, the importance of these splice isoforms is poorly understood. Here we report a critical role of E6^E7, a novel isoform containing the 41 N-terminal amino acid (aa) residues of E6 and the 38 C-terminal aa residues of E7, in the regulation of...

HeLa nucleic acid contamination in The Cancer Genome Atlas leads to the 1 misidentification of HPV18 2 3 Paul G. Cantalupo, Joshua P. Katz, James M. Pipas# 4 5 Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA 6 7 Running title: HeLa contamination in TCGA misidentifies HPV18 8 9 # Corresponding author: James Pipas, [email protected]. 10 11 Abstract word cou...

The human papillomaviruses (HPVs) are associated with specific benign and malignant lesions of the skin and mucosal epithelia. Cloned viral DNAs from HPV types 6b, 16, and 18 associated with different pathological manifestations of genital neoplasia in vivo were introduced into primary human cervical epithelial cells by electroporation. Cells transfected with HPV16 or HPV18 DNA acquired indefin...

Introduction Peroxiredoxin 3 (PRX3) is a member of PRX family with antioxidant functions by scavenging hydrogen peroxide. Since the development cervical cancer causally linked to high-risk human papillomavirus (HPV) that induces oxidative stress, we conducted present study investigate response PRX3 HPV infection. Material and methods This included fifty-six patients invasive squamous sixty cont...