Mechanisms about regulation of mRNAs, microRNAs (miRNAs), and long non-coding RNAs (lncRNA) in cervical lymph node metastatic laryngeal squamous cell carcinoma (LSCC) remained unclear. Two sets of three microarrays of mRNA, miRNA and lncRNA in fresh surgical LSCC samples were established, respectively from three paired tumor and adjacent normal mucosa epithelial tissues in cN + (Positive metast...

The long non-coding RNA (lncRNA) RUSC1-AS1 has been reported to be dysregulated in the progression of many cancers. Also, had detected highly expressed laryngeal squamous cell carcinoma and breast cancer cells, suggesting that may a biomarker for However, biological role regulatory mechanism hepatocellular (HCC) remain unknown. In this study, we found was upregulated HCC tissues predicted unfav...

We investigated the transcriptional mechanism underlying lung cancer development. RNA sequencing analysis was performed on blood samples from lung cancer cases and healthy controls. Differentially expressed microRNAs (miRNAs), circular RNAs (circRNAs), mRNAs (genes), and long non-coding RNAs (lncRNA) were identified, followed by pathway enrichment analysis. Based on miRNA target interactions, a...

Background: Accumulating evidence suggests that postmenopausal osteoporosis (PMOP) is a common chronic systemic metabolic bone disease, but its specific molecular pathogenesis remains unclear. This study aimed to identify novel genetic diagnostic markers for PMOP. Methods: In this paper, we combined three GEO datasets differentially expressed genes (DEGs) and performed functional enrichment ana...

Intervertebral disc degeneration (IDD) leads to low back pain (LBP). This study aimed determine the regulation of IDD by competing endogenous RNAs (ceRNAs). We obtained GSE63492, GSE124272, and GSE129789 datasets from Gene Expression Omnibus database. The changes long non-coding (lncRNAs), microRNAs (miRNAs), mRNAs in were characterized. significantly changed subjected protein-protein interacti...

Recent research raised the possibility that some viral microRNAs (miRNAs) may function as orthologs of cellular miRNAs. In the present work, to study the functional orthologous relationships of viral and cellular miRNAs, we first constructed a dual-fluorescent protein reporter vector system for the easy determination of miRNA function. By expressing the miRNAs and the indicator and internal con...

The aim of the current study was to analyze the differential microRNA (miRNA) expression profiles of human epidermal stem cells (ESCs) and differentiated keratinocytes. Enzyme digestion was used in combination with rapid adhesion to collagen IV to isolate primary human ESCs and differentiated keratinocytes, from which total RNA was extracted. Fluorescence labeling, microarray hybridization and ...

Elite controllers maintain HIV-1 viral loads below the limit of detection. The mechanisms responsible for this phenomenon are poorly understood. As microRNAs (miRNAs) are regulators of gene expression and some of them modulate HIV infection, we have studied the miRNA profile in plasma from HIV elite controllers and chronically infected individuals and compared against healthy donors. Several mi...

BACKGROUND Metastasis is the major cause of cancer-related death in patients with gastric cancer, and aberrant expression of various microRNAs (miRNAs) is associated with cancer metastasis. METHODS Profiling of differentially expressed miRNAs was performed in three cases of primary gastric cancer and the corresponding metastatic lymph node tissues. Then, the five most altered miRNAs were furt...