PURPOSE Maintenance of avascularity of the normal cornea and control of neovascularization during wound healing depend on a balance of angiogenic and antiangiogenic factors. The purpose of this paper is to determine the ability of corneal cells to convert plasminogen to angiostatins and to compare these products with those made by intact corneas. METHODS RT-PCR was performed using plasminogen...

PURPOSE Corneal endothelial cells in humans do not replicate to any meaningful extent. Diminishing density of the cell monolayer with age and in the disease states is a major cause of loss of corneal transparency. This study was conducted to test the hypothesis that overexpression of the transcription factor E2F2 results in replication in nonproliferating human corneal endothelial cells. METH...

BACKGROUND Though the transplantation of in vitro expanded human corneal endothelial precursors in animal models of endothelial damage by injecting into the anterior chamber has been reported, the practical difficulties of accomplishing such procedure in human patients have been a hurdle to clinical translation. Here we report the successful transplantation of in vitro expanded human corneal pr...

PURPOSE To establish the immortalized human corneal endothelial cell line (IHCEn) by transducing human papilloma virus (HPV) 16 E6/E7 oncogenes, and to identify their characteristics when cultivated on a lyophilized human amniotic membrane (LAM). METHODS Primary human corneal endothelial cells (PHCEn) were infected using a retroviral vector with HPV 16 E6/E7, and transformed cells were clonal...

PURPOSE Several growth factors, including nerve growth factor (NGF) and vascular endothelial growth factor (VEGF), play an important role in the homeostasis of the ocular surface. The involvement of both these growth factors in the pathophysiology of intraocular tissues has been extensively investigated. Despite the expression of NGF receptors by corneal endothelium, to date the role of NGF on ...

PURPOSE To determine if aquaporin-1 expression is decreased in human and mouse corneal endothelial dysfunction. METHODS Immunohistochemistry with anti-aquaporin 1 antibody and confocal microscopy were used to study a case series of human corneal specimens, and a mouse model of corneal endothelial injury was created with injection of sterile hot water into the anterior chamber with a 33 gauge ...

PURPOSE The corneal endothelium is a monolayer of cells in the posterior cornea that is responsible for maintaining a clear cornea. Corneal endothelial cells may be induced to divide, but it has been held that they do not divide in the normal cornea of an adult human. Some studies have suggested that a stem cell population for the corneal endothelium exists. This population could give rise to m...

Human cornea is a highly organized transparent tissue and is well determined by its functional layers as shown in (Figure 1). The posterior layer of the cornea is the endothelium which is a physiologically important monolayer of cells. Human Corneal Endothelium (CE) plays a major role in maintaining the corneal transparency, thickness and hydration [1,2]. As CE dysfunction is the second leading...

purpose : to compare central corneal thickness (cct), corneal endothelial cell density, and lens capsule thickness in normotensive patients with and without pseudoexfoliation syndrome (pxs) methods : this was a prospective, comparative, descriptive study. normotensive candidates for cataract surgery with (study group) and without (control group) pxs were enrolled in the study. cct and corneal e...