many investigations have proved relations between abo blood groups with some diseases and factor viii and von willebrand level in plasma. in this study we investigated a relation between abo blood groups and factor viii and ix inhibitors in 102 patients with haemophilia a and 48 patients with haemophilia b. the assay of inhibitor was done by bethesda method. there were no relation between abo b...

This study applied Human Factors Intervention Matrix (HFIX) framework and Analytic Hierarchy Process (AHP) to develop human errors intervention strategy. Our AHP questionnaire,designed based on the 31 incident scenarios that were taken place between 2009 and 2011 and included 72 safety recommendations to reduce specific human errors, was distributed to commercial airlines related personnel and ...

INTRODUCTION Gene therapy could prevent bleeding in hemophilia. However, antibodies could inhibit coagulation, while cytotoxic T lymphocytes could destroy modified cells. The immaturity of the newborn immune system might prevent these immune responses from occurring after neonatal gene therapy. MATERIALS AND METHODS Newborn dogs, cats, or mice were injected intravenously with a retroviral vec...

Background Plants constitute promising systems for the alternative production of valuable recombinant proteins. Recently, ELELYSOTM, an enzymatic drug developed by the Israeli company Protalix Biotherapeutics to treat Type 1 Gaucher’s disease, became the first plant-derived therapeutic product to reach marketable status [1]. This confirms the inherent potential of plant systems for the large-sc...

We have determined the 2905 nucleotide sequence of the rhesus macaque factor IX complementary DNA (cDNA) and found it to be greater than 95% identical to that of the human factor IX cDNA. The cDNA has a large 3' untranslated region like the human cDNA, but unlike the human cDNA has two polyadenylation sites 224 nucleotides apart that are used for transcription of the messenger RNA. The deduced ...

Mouse primary skin fibroblasts were infected with a recombinant retrovirus containing human factor IX cDNA. Bulk infected cells capable of synthesizing and secreting biologically active human factor IX protein were embedded in collagen, and the implant was grafted under the epidermis. Sera from the transplanted mice contain human factor IX protein for at least 10-12 days. Loss of immunoreactive...

Hemophilia A has an incidence of 1 in 5000 males and is due to deficiency of hFVIII. The mainstay of treatment is intravenous injection of hFVIII protein prophylactically or during bleeding, which is expensive and inconvenient. Identification of alternative treatments would be a very important advance. Gene therapy for hemophilia A involves transfer of a FVIII gene into cells that can secrete f...

in this study, anion-exchange chromatography was used to purify factor vii and factor ix from prothrombin complex (ppsb), which contains coagulation factors ii, vii, ix and x. for this purpose, deae-sepharose cl-6b gel , pharmacia column xk-26 , high flow rate and two stepwise gradients with phosphate citrate buffer were used. the yield of the two lyophylized products, factor vii and factor ix ...