The effects of human recombinant erythropoietin (rEpo) in the presence of other stimulators on the growth of clonogenic leukemic blast cells from ten Japanese patients with acute myeloblastic leukemia were studied with an in vitro leukemic blast colony assay in methylcellulose culture. With the addition of rEpo alone, no leukemic blast colony formation was stimulated in any of the cases examine...

ABBREVIATIONS. USPHS, US Public Health Service; IDSA, Infectious Diseases Society of America; HIV, human immunodeficiency virus; PCP, Pneumocystis carinii pneumonia; TE, toxoplasmic encephalitis; MAC, Mycobacterium avium complex; AIDS, acquired immunodeficiency syndrome; TB, tuberculosis; TMP/ SMZ, trimethoprim/sulfamethoxazole; BI, bacterial infections; TST, tuberculin skin test; PPD, purified...

Recombinant gibbon interleukin-3 (IL-3) is a multilineage hematopoietic colony-stimulating factor (CSF) that recently was cloned and found to be highly homologous with human IL-3. Gibbon IL-3, as well as human granulocyte-CSF (G-CSF) and human granulocyte-macrophage CSF (GM-CSF), stimulated normal human bone marrow cells to form myeloid colonies in soft agar in a sigmoidal dose-response manner....

a self-regulated high-copy number plasmid containing chloramphenicol resistant gene, for the production of recombinant proteins under the regulation of bacteriophage ?pl promoter, was constructed. the designed 5024 base pair expression plasmid contained a heat sensitive repressor ci857 coding gene to regulate the function of ?pl promoter under heat shock induction. using the constructed vector,...

Human granulocyte-colony stimulating factor (hG-CSF) cDNA was expressed in the methylotrophic yeast Pichia pastoris under the control of the alcohol oxidase (AOX1) promoter. An expression vector for hG-CSF secretion was constructed using vector pPIC9. Higher levels of hG-CSF was obtained using a P. pastoris Mut+ (methanol utilization fast) phenotype. The effects of environmental factors such as...

bakground: granulocyte colony-stimulating factor (g-csf) is a cytokine that stimulates hematopoiesis and induces proliferation and differentiation of granulocyte progenitor cells as well as production of bone marrow neutrophilic granulocyte colonies.  nowadays, human recombinant g-csf(hr g-csf) is used for the treatment of chemotherapy- and radiotherapy-induced neutropenia, and also in patients...

Interleukin-7 (IL-7) is an important growth factor in B and T lymphopoiesis in mouse and human, whereas IL-7 has been regarded to lack proliferative effects on cells within the myeloid lineage. However, we have recently reported that IL-7 potently can enhance colony stimulating factor (CSF)-induced myelopoiesis from primitive murine hematopoietic progenitors, showing a novel role of IL-7 in ear...

Hematopoietic stem/progenitor cells (HSPCs) which give rise to different blood cell types are present within the bone marrow microenvironment, especially in flat bones such as skull, vertebrae, pelvis and chest. Interacting factors such as stromal derived factor-1/CXCR4, very late antigen-4/vascular cell adhesion molecule-1, Lymphocyte function-associated antigen-1/ intercellular adhesion molec...

objective(s): granulocyte-colony stimulating factor (g-csf) is used in clinical practice for the treatment of neutropenia and to stimulate generation of hematopoietic stem cells in bone marrow donors. in the present study, the ability of g-csf in mobilizing exogenous bone marrow stem cells (bmscs) from peripheral blood into the brain was tested. we for the first time injected a small amount of ...