Using the electron spin resonance/spin trapping system, 4-pyridyl 1-oxide N-tert-butylnitrone (4-POBN)/ethanol, hydroxyl radical was detected as the alpha-hydroxyethyl spin trapped adduct of 4-POBN, 4-POBN-CH(CH3)OH, from phorbol 12-myristate 13-acetate-stimulated human neutrophils and monocytes without the addition of supplemental iron. 4-POBN-CH(CH3)OH was stable in the presence of a neutroph...

Since the various membrane abnormalities of sickle erythrocytes might result from excessive accumulation of oxidant damage, we have measured the generation of superoxide, peroxide, and hydroxyl radical by normal and sickle erythrocytes using assays involving reduction of cytochrome c, aminotriazole inhibition of catalase, and methane evolution from dimethyl sulfoxide, respectively. Compared wit...

Iron chlorin e6 (FeCe6) has recently been proposed to be potentially antimutagenic and antioxidative. However, the antioxidant property of FeCe6 has not been elucidated in detail. In this study, we investigated the ability of FeCe6 to scavenge hydroxyl radical and to protect biomolecules and mammalian cells from oxidative stress-mediated damage. In electron spin resonance (ESR) experiments, FeC...

The extent of oxidoreduction of the 3 alpha-, 7 alpha- and 12 alpha-hydroxyl groups in bile acids during the enterohepatic circulation in man was studied with the use of [3 beta-3H]-labeled deoxycholic acid and cholic acid, [7 beta-3H]-labeled cholic acid, and [12 beta-3H]-labeled deoxycholic acid and cholic acid. Each [3H]-labeled bile acid was given per os to healthy volunteers, together with...

Curcumin is extracted from the rhizomes of the traditional Chinese herb Curcuma longa. Our previous study indicated curcumin was able to function as a sonosensitizer. Hydroxyl acylated curcumin was synthesized from curcumin to eliminate the unstable hydroxy perssad in our group. The potential use of Hydroxyl acylated curcumin as a sonosensitizer for sonodynamic therapy (SDT) requires further ex...

Sulfuric acid ([3-(3-silicapropyl)sulfanyl]propyl]ester (SASPSPE) is employed as a recyclable catalyst for the silylation of hydroxyl groups. A good range of primary, secondary alcohols and phenolic hydroxyl groups were effectively converted into their corresponding trimethylsilyl ethers with hexamethyldisilazane in the presence of catalytic amounts of SASPSPE at room temperature with short rea...

The specificity of the enzyme purified from TZ-infected Escherichia coli which catalyzes the phosphorylation of 5’hydroxyl ends of ribonucleic acid and deoxyribonucleic acid has been examined. The enzyme also catalyzes the phosphorylation of 5’-hydroxyl groups of polynucleotides as well as 3’-mononucleotides. There is no detectable phosphorylation of nucleosides and adenosine 2’-phosphate is vi...

The specificity of the enzyme purified from TZ-infected Escherichia coli which catalyzes the phosphorylation of 5’hydroxyl ends of ribonucleic acid and deoxyribonucleic acid has been examined. The enzyme also catalyzes the phosphorylation of 5’-hydroxyl groups of polynucleotides as well as 3’-mononucleotides. There is no detectable phosphorylation of nucleosides and adenosine 2’-phosphate is vi...

An enzyme, 5’-hydroxyl polyribonucleotide kinase, which catalyzes the phosphorylation of 5’-hydroxyl ends of RNA in the presence of ATP, has been isolated from extracts of HeLa cell nuclei. The kinase requires a divalent cation (Mg’+ or Mn’+) for activity, has an alkaline pH optimum, and is sensitive to the sulfhydryl antagonist N-ethylmaleimide. 5’-Hydroxyl terminated polydeoxyribonucleotides ...

An enzyme, 5'-hydroxyl polyribonucleotide kinase, which catalyzes the phosphorylation of 5'-hydroxyl ends of RNA in the presence of ATP, has been isolated from extracts of HeLa cell nuclei. The kinase requires a divalent cation (Mg2+ or Mn2+) for activity, has an alkaline pH optimum, and is sensitive to the sulfhydryl antagonist N-ethylmaleimide. 5'-hydroxyl terminated polydeoxyribonucleotides ...