نتایج جستجو برای: ibv
تعداد نتایج: 499 فیلتر نتایج به سال:
Virus-induced apoptosis and viral mechanisms that regulate this cell death program are key issues in understanding virus-host interactions and viral pathogenesis. Like many other human and animal viruses, coronavirus infection of mammalian cells induces apoptosis. In this study, the global gene expression profiles are first determined in IBV-infected Vero cells at 24 hours post-infection by Aff...
In the United States, more than 90% of chicken meat is produced in the southeastern states, and most egg production resides in the eastern half of the country and Texas. Several molecular epidemiological studies have indicated that most infectious bronchitis (IB) virus (IBV) isolates obtained from outbreaks of respiratory disease in these regions correspond to Ark-type IBV in spite of extensive...
Influenza A and B viruses show clear differences in their host specificity and pandemic potential. Recent studies have revealed that the host protease TMPRSS2 plays an essential role for proteolytic activation of H1, H3, and H7 subtype strains of influenza A virus (IAV) in vivo. IAV possessing a monobasic cleavage site in the haemagglutinin (HA) protein replicates poorly in TMPRSS2 knockout mic...
A simple gold-immunochromatographic assay (GICA) based on indirect reaction format was developed for rapid detection of antibodies against avian infectious bronchitis virus (IBV). The detection time of IBV IgG by the GICA was less than 10 min, whereas the HI test and the enzyme-linked immunosorbent assay (ELISA) require 1-2 hr. Reference sera against newcastle disease virus, infectious bursal d...
We report here the identification of a 24-kDa polypeptide in IBV-infected Vero cells by immunoprecipitation with a region-specific antiserum raised in rabbits against the IBV sequence encoded between nucleotides 10,928 and 11,493. Coexpression, deletion, and mutagenesis studies have demonstrated that this protein is encoded by ORF 1a from nucleotide 10,915 to 11,544 and is released from the 1a ...
Infectious proventriculitis syndrome is at times a serious and ongoing problem causing significant financial losses. From the obtained results in the present study, it could be concluded that proventriculitis can be transmitted by oral inoculation of homogenates produced from proventriculi of birds with proventriculitis. Experimentally inoculated broilers were seroconverted to ARV, IBV and IBDV...
The avian coronavirus (AvCoV) infectious bronchitis virus (IBV) is a major poultry pathogen. A characteristic feature of IBV is the occurrence of many different strains belonging to different serotypes, which makes a complete control of the disease by vaccinations a challenging task. Reasons for differences in the tissue tropism and pathogenicity between IBV strains, e.g. a predilection for the...
Here we introduce a culture system for the isolation, passaging and amplification of avian tracheal epithelial (ATE) cells. The ATE medium, which contains chicken embryo extract and fetal bovine serum, supports the growth of ciliated cells, goblet cells and basal cells from chicken tracheas on fibronectin- or matrigel-coated dishes. Non-epithelial cells make up less than 10% of the total popula...
Despite the existence of an active vaccination program, recently emerged strains of nephropathogenic infectious bronchitis virus (IBV) in Korea have caused significant economic losses in the poultry industry. In this study, we assessed the pathogenic and antigenic characteristics of a K-IIb type field strain of IBV that emerged in Korea since 2003, such as Kr/Q43/06. Specific pathogen free 1-we...
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