Analyzing N&B using AVI movies I converted the AVI movie (a collection of bitmaps) to a format that SimFCS can read. This is done reading the movie using ImageJ and then saving the file as raw data. The raw data shows that the movie is 150x150 pixels and contains 900 frames. I read the raw data using the following screen and analyze a part of it in the 128x128 format. There is a fast initial bl...

Recent advances in biological imaging techniques and the enormous amount of data they generate call for the development of computational tools for efficient and reliable high-throughput analysis. Several software applications with this functionality are available, and one of the most commonly used is ImageJ. Here, we present two independent macros (WH_NJ and SA_NJ) for automating and facilitati...

Counting cells and colonies is an integral part of high-throughput screens and quantitative cellular assays. Due to its subjective and time-intensive nature, manual counting has hindered the adoption of cellular assays such as tumor spheroid formation in high-throughput screens. The objective of this study was to develop an automated method for quick and reliable counting of cells and colonies ...