After studying the effect of function on the strength of tendon repairs in dogs, Mason and Allen (1941) concluded that immobilisation was necessary for two or three weeks. This had been taught by Mayer (1938) and was followed by Koch (1943) and Bunnell (1948). Since then Miller (1942), Farquharson (1950), Rank and Wakefield (1953), Nemethi (1956), Kelly (1959), Flatt (1959), and Clarkson and Pe...

A set of supports were screened for the immobilization of a partially purified extract of β-glucosidase from Aspergillus sp. These supports, namely, Eupergit, Amberlite, alginate, gelatin, polyvinyl alcohol- (PVA-) based matrices (Lentikats), and sol-gel, have proved effective for the implementation of some other enzyme-based processes. The initial criterion for selection of promising supports ...

In this review, we detail the efforts performed to couple the purification and the immobilization of industrial enzymes in a single step. The use of antibodies, the development of specific domains with affinity for some specific supports will be revised. Moreover, we will discuss the use of domains that increase the affinity for standard matrices (ionic exchangers, silicates). We will show how ...

Hydroxynitrile lyases are a versatile group of enzymes that are applied both in the laboratory and on an industrial scale. What makes them particularly interesting is that to date five structurally unrelated categories of hydroxynitrile lyases have been discovered. Given their great importance they have often been immobilised utilising many different methodologies. Therefore the hydroxynitrile ...

Most of the supports for enzymeimmobilization are artificial materials, and chemical reactions are also required to immobilize the enzymes in most cases.1] These support materials and chemicals for immobilization must not be allowed to contaminate final products, especially foods or drugs. The requirements to achieve this are tedious, costly, and may have the wrong effects on enzyme activities....

The immobilization and differential pulse anodic voltammetry (DPAV) of a 20-mer oligonucleotide related to the human interleukine-2 (hIL-2) using renewable pencil graphite electrode (PGE) is described. The influences of electrochemical pretreatment of PGE on the ability of the electrode in hIL-2 adsorption, and conditions of hiIL-2 immobilization on PGE including immobilization potential and ti...

INTRODUCTION Vital signs remain important clinical indicators in the management of trauma. Tissue injury and ischemia cause tachycardia and hypertension, which are mediated via the sympathetic nervous system (SNS). Spinal immobilization is known to cause discomfort, and it is not known how this might influence the SNS and contribute to abnormal vital signs. Hypothesis This study aimed to establ...

The main objective of the immobilization of enzymes is to enhance the economics of biocatalytic processes. Immobilization allows one to re-use the enzyme for an extended period of time and enables easier separation of the catalyst from the product. Additionally, immobilization improves many properties of enzymes such as performance in organic solvents, pH tolerance, heat stability or the functi...

Immobilization of biomolecular probes to the sensing substrate is a critical step for biosensor fabrication. In this work we investigated the phosphate-dependent, oriented immobilization of DNA to hafnium dioxide surfaces for biosensing applications. Phosphate-dependent immobilization was confirmed on a wide range of hafnium oxide surfaces; however, a second interaction mode was observed on mon...