The study of the stability enhancement of a peroxidase immobilized onto mesoporous silicon/silica microparticles is presented. Peroxidases tend to get inactivated in the presence of hydrogen peroxide, their essential co-substrate, following an auto-inactivation mechanism. In order to minimize this inactivation, a second protein was co-immobilized to act as an electron acceptor and thus increase...

The biotechnological applications use stable and eco-friendly biocatalysts, with good operational properties, reusable easily separable. An efficient method of improving the stability enzymes as biocatalysts may be immobilization. immobilization research objectives are obtaining versatile insoluble enzyme preparations high enzymatic activity, capable catalyzing reactions their natural unnatural...

Nanostructured materials along with an added value of polymers-based support carriers have gained high interest and considered ideal for enzyme immobilization. The recently emerged nanoscience interface in the form nanostructured combined immobilized-enzyme-based bio-catalysis has now become research development frontiers advance applied engineering. With involvement nanoscience, various polyme...

Immobilization of enzymes is one of the most promising methods in enzyme performance enhancement, including stability, recovery, and reusability. However, investigation of suitable solid support in enzyme immobilization is still a scientific challenge. Polyethersulfone (PES) and aminated PES (PES-NH2) were successfully synthesized as novel materials for immobilization. Membranes with various po...

Biocatalysis with free enzymes may not be economically viable due to the complexity of their recovery in the reaction medium. With the objective of enabling the total activity recovery yield of biocatalyst and to improve the operational characteristics, have been applied enzyme immobilization techniques. The main interest in the enzyme immobilization is to obtain a catalyst whose activity and s...

Among the micropollutants identified in environment, non-steroidal anti-inflammatory drugs (NSAIDs) dominate more and often. This is due to both high consumption low efficiency of biological wastewater treatment plants, where initial transformation NSAIDs most often takes place. The solution problem may be using preparations supporting activated sludge sewage plants biodegradation NSAIDs. There...

The cost of biodiesel production relies on feedstock cost. Edible oil is unfavorable as a biodiesel feedstock because of its expensive price. Thus, non-edible crop oil, waste oil, and microalgae oil have been considered as alternative resources. Non-edible crop oil and waste cooking oil are more suitable for enzymatic transesterification because they include a large amount of free fatty acids. ...

Immobilization has emerged since last decade as a very powerful tool to improve almost all enzyme properties like stability, activity, specificity and selectivity, and reduction of inhibition. The immobilization may help to solve some of the problems of enzymes as industrial biocatalysts like enzyme recovery for reuse. In the present investigation A.niger was isolated from soil and identified b...

Lipase from Thermomyces lanuginosus (TLL) has been immobilized on Purolite Lifetech® ECR8806F (viz. methacrylate macroporous resin containing octadecyl groups, designated as C18-TLL), and the enzyme performance compared to that of octyl-agarose, agarose C8-TLL. The hydrolytic activity versus p-nitrophenol butyrate decreased significantly, a lower extent S-methyl mandelate (more than twofold), w...