Several clinical laboratory methods using enzymes as reagents now may utilize immobilized enzymes. These are enzymes attached to solid surfaces by adsorption, covalent binding, cross linking or similar means. Immobilized enzymes are widely used presently in convenient tests for urine glucose and galactose or blood glucose and urea, and in serum glucose or urea determinations by automated method...

Abstract Purpose of Review In the presented review, we have summarized recent achievements on use immobilized oxidoreductases for biodegradation hazardous organic pollutants including mainly dyes, pharmaceuticals, phenols, and bisphenols. order to facilitate process optimization achievement high removal rates, effect various conditions has been highlighted discussed. Recent Findings Current rep...

Enzymes serve as biocatalysts for innumerable important reactions, however, their application has limitations, which can in many cases be overcome by using appropriate immobilization strategies. Here, a new support for immobilizing enzymes is proposed. This hybrid organic-inorganic support is composed of chitosan-a natural, nontoxic, biodegradable, and edible biopolymer-and sodium polyphosphate...

The overall objective of this research is to enhance the bioactivity of cellulase enzymes as well as the stability of the enzymes to increase it reusability through immobilization of the enzymes in the microenvironment of porous inorganic-organic hybrid sol-gel polymers. Recently, we have discovered that the microenvironment of these sol-gel polymers markedly increases the bioactivity of immobi...

In the last ten years great advances have been made in elucidating the properties of immobilized enzymes, which have already found practical applications in chemical analysis as well as in large scale industrial processes. The term "immobilized enzymes" embraces a variety of systems in which one or more enzymes are sequestered in gels or microcapsules, covalently bound or adsorbed to solid supp...

In the last ten years great advances have been made in elucidating the properties of immobilized enzymes, which have already found practical applications in chemical analysis as well as in large scale industrial processes. The term "immobilized enzymes" embraces a variety of systems in which one or more enzymes are sequestered in gels or microcapsules, covalently bound or adsorbed to solid supp...

While enzymes are valuable tools in many fields of biotechnology, they are fragile and must be protected against denaturing conditions such as unfavorable solution pH. Within living organisms, chaperonins help enzymes fold into their native shape and protect them from damage. Inspired by this natural solution, mesoporous silica SBA-15 with different pore diameters is synthesized as a support ma...

Numerous types of nanoparticles and nanocomposites have successfully been employed for the immobilization and stabilization of amylolytic enzymes; α-amylases, β-amylases, glucoamylases and pullulanases. Nano-support immobilized amylolytic enzymes retained very high activity and yield of immobilization. The immobilization of these enzymes, particularly α-amylases and pullulanases, to the nanosup...

a-amylase was immobilized on alumina via adsorption. The support and the immobilized enzymes were characterized using XRD, IR spectra and N2 adsorption studies. The efficiency of immobilized enzymes for starch hydrolysis was tested in a batch reactor. The effect of two different calcination temperatures on properties of the support as well as on immobilization was studied. From XRD, IR and N2 a...