A specific and very sensitive dot-immunobinding assay for the detection and enumeration of the bioleaching microorganism Thiobacillus ferrooxidans was developed. Nitrocellulose spotted with samples was incubated with polyclonal antisera against whole T. ferrooxidans cells and then in I-labeled protein A or I-labeled goat antirabbit immunoglobulin G; incubation was followed by autoradiography. S...

IgG antibody to Mycobacterium tuberculosis from the sera of patients with 'definite' pulmonary tuberculosis (PT) was isolated and coupled with Cyanogen bromide-Sepharose 4B. Using an immunoabsorbent affinity chromatography, 14 kDa antigen was recovered from the culture filtrates of M. tuberculosis. With this mycobacterial antigen, a dot immunobinding assay (Dot-Iba) was developed for the detect...

In the present prospective study, a dot immunobinding assay (Dot-Iba) was standardized to measure the circulating mycobacterial antigen in cerebrospinal fluid (CSF) specimens for the laboratory diagnosis of tuberculous meningitis (TBM). Immunoglobulin G antibody specific for Mycobacterium tuberculosis in a CSF specimen from a patient with culture-proven TBM was isolated and was coupled with act...

Neocortical decreases in synaptic density correlate significantly with the cognitive impairment seen in Alzheimer disease. Recently available monoclonal antibodies (MAb) have made possible the highly specific and sensitive detection of synapse-associated proteins in immunocytochemical and immunochemical techniques. We describe a simple yet highly sensitive dot-immunobinding assay for relative q...

By changing the N-donor groups of benzonitrile-based reactants, three novel isopolymolybdate-based compounds, [Cu(DBIBA)3][δ-HMo8O26] (1), [Cu(H2O)(DIBA)(DIBAH)(γ-HMo8O26)]·2H2O (2), and [CuI(DTBA)(Mo2O6)] (3) (HDBIBA = 3,5-di(benzoimidazol-1-yl)benzoic acid, HDIBA = 3,5-di(1H-imidazol-1-yl)benzoic acid and HDTBA = 3,5-di(1,2,4-triazol-1-yl)benzoic acid) were synthesized, in which the benzene c...