INTRODUCTION Conventional serology was used for the detection of Trypanosoma cruzi infection, with diverse sensitivity and specificity results. Due to the number of samples with doubtful results, it is necessary to develop additional confirmation tests such as the immunoblot. OBJECTIVE The aim of this study was identify major immunogenic proteins of T. cruzi isolate and establish criteria for...

A quantitative urea-based amyloid b (Ab)-sodium dodecyl sulfate-polyacrylamide gel electrophoresis with Western immunoblot (Ab-SDS-PAGE/immunoblot) reveals highly conserved and disease-specific Ab peptide patterns (Ab 1-37, 1-38, 1-39, 1-40, 1-42) in Alzheimer’sdisease (AD)patientsandnondementedcontrols. For further standardization of this method, we analyzed cerebrospinal fluid (CSF) of eight ...

The aim of the study was to investigate whether patients with Aspergillus-induced lung disease can be monitored by immunoblot analysis to detect antibodies to Aspergillus fumigatus (Af). Immunoblotting was performed by incubating 57 longitudinally collected sera from 13 patients on nitrocellulose sheets, blotted with Af antigen, separated by sodium dodecyl sulphate-polyacrylamide gel electropho...

An immunoblot procedure for the strain-specific quantitative analysis of commercial Rhizobium inoculants was developed. The technique greatly reduced the time required for inoculant analysis. Correlation between immunoblot analysis and traditional plant nodule grow-out most-probable-number techniques was r = 0.90 for 16 commercial alfalfa inoculants tested.

The serological response to Aspergillus fumigatus was investigated in patients with pulmonary aspergillosis using the immunoblot technique. Antibody was detected against nine components of the fungus, ranging in molecular weight from 88 000 to 33 000 daltons. Antibody to a 40 000 dalton component was present in most patients (13 of 16). In surviving patients it was high (two with aspergilloma) ...