OBJECTIVE To find the prevalence of lupus nephritis, delineate its clinical, immunological and therapeutic characteristics and compare them with the data worldwide. PATIENTS AND METHODS Between 1985 and 2001, 198 patients with SLE fulfilling the clinical and laboratory criteria of the American Rheumatism Association (ARA) admitted to the hospital were studied by means of a retrospective revie...

Background: Immune mediated vesiculobullous diseases can be diagnosed by close attention to the clinical details, histological confirmation and the clinical setting of the blistering. Confirmatory studies such as immunofluorescence will help in diagnosis of cases where histopathological features alone are not diagnostic. To evaluate clinical findings in association with histopathological findin...

Mutations in breast cancer tumor susceptibility genes, BRCA1 and BRCA2, predispose women to early onset breast cancer and other malignancies. The Brca genes are involved in multiple cellular processes in response to DNA damage including checkpoint activation, gene transcription, and DNA repair. Biochemical interaction with the recombinational repair protein Rad51 (Scully, R., Chen, J., Ochs, R....

BACKGROUND Dengue virus (DENV) enters cells via endocytosis, traffics to perinuclear (PN) region, the site of morphogenesis and exits by exocytosis. This study aims to understand the role of dynamin II, endosomes, microtubules (MT) and dynein in the early events of DENV replication. FINDINGS Using double immunoflourescence labelling of DENV-2 infected BHK-21 cells it was observed that the sur...

OBJECTIVE In order to retain an undifferentiated pluripotent state, embryonic stem (ES) cells have to be cultured on feeder cell layers. However, use of feeder layers limits stem cell research, since experimental data may result from a combined ES cell and feeder cell response to various stimuli. MATERIALS AND METHODS In this experimental study, a buffalo ES cell line was established from in ...

The occurrence of vimentin, a specific intermediate filament protein, has been studied by immunoflourescence microscopy in tissue of adult and embryonic brain as well as in cell cultures from nervous tissue. By double imminofluorescence labeling, the distribution of vimentin has been compared with that of subunit proteins of other types of intermediate filaments (glial fibrillary acidic [GFA] p...

Nm23-H1 has been identified as a metastasis suppressor gene, but its protein interactions have yet to be understood with any mechanistic clarity. In this study, we evaluated the proteomic spectrum of interactions made by Nm23-H1 in 4T1 murine breast cancer cells derived from tissue culture, primary mammary tumors, and pulmonary metastases. By this approach, we identified the actin-severing prot...

Abstract Study question Does verteporfin administration effect on proliferation, survival and migration of TCam-2 human seminoma cells? Summary answer Verteporfin may be a promising anticancer agent for cells via inhibition cell viability, stimulation apoptosis. What is known already Seminoma germ tumor the testis first seminoma-derived line that retains many characteristic traits seminoma. The...

a r t i c l e i n f o Titanium surfaces were designed, produced, and evaluated for levels of osseointegration into the femurs of rabbits. A total of 36 Ti-6Al-4V pins (15 mm length, 1.64 mm diameter) were prepared into three experimental groups. These were designed to test the effects of osseointegration on laser grooved, RGD coated, and polished control surfaces, as well as combined effects. C...

Skeletal muscle is the mammalian body's largest protein resevoir, serving the critical role of mobilizing proteins during acute conditions, such as trauma and starvation, and chronic conditions, such as AIDS, cancer, and muscular dystrophy. When proteins are mobilized in excess of physiological needs, muscle wasting ensues. With advances in the treatment of the primary effects of these conditio...