In a clinical study of 190 men with non-gonococcal urethritis, Chlamydia trachomatis inclusions were sought in cycloheximide-treated McCoy cells by an indirect immunofluorescent antibody technique. The method was consistently reliable over a period of two years, and the results were obtained within 24 hours of a patient's attendance. The results correlated with those obtained by Giemsa staining...

Microtubule network provides many intracellular microbes with an efficient way to move within host cells. Orientia tsutsugamushi move from the cell periphery to the microtubule organizing center (MTOC) by dynein-dependent mechanism. In this study, we investigated the role of microtubule on the growth of O. tsutsugamushi. The treatment of infected cells with taxol as well as daunomycin enhanced ...

BACKGROUND Venous malformation (VM) is the most common form of vascular malformation, consisting of a network of thin-walled ectatic venous channels with deficient or absent media. This study investigated the expression of the components of the renin-angiotensin system (RAS), namely, (pro)renin receptor (PRR), angiotensin-converting enzyme (ACE), angiotensin II receptor 1 (ATIIR1), and angioten...

High-speed (80,000 X g) pellets of plasma from leukemic patients and from normal individuals were used to prepare antisera in rabbits. The leukemic plasma pellets contained virus-like particles as determined by electron microscopy, whereas similar particles were not observed in the normal pellets. Globulin fractions from six antisera to normal pellets (NPA) and six antisera to leukemic pellets ...

Immunofluorescent techniques have been used extensively to detect the presence of antinuclear factors (ANF) in sera from patients with systemic lupus erythematosus and related disorders. Furthermore, morphological differences in the character of nuclear immunofluorescence have been considered to be of clinical as well as immunological significance (Casals, Friou, and Teague, 1963; Burnham, Fine...

A method is described for preparation of polyvalent antigens for use in rapid screening for immunofluorescent antibodies to Lassa, Marburg, and Ebola viruses. The technique uses mixtures of specifically infected Vero cells placed on Teflon-templated microscopy slides. It was found to be as sensitive as the use of monovalent antigens for detection and quantitation of antibodies to these highly h...