In order to classify the underlying disorder in four patients with biopsy-proven amyloidosis without overt monoclonal gammopathy, the cytoplasmic immunoglobulin (Ig) distribution of bone marrow plasma cells was evaluated using direct immunofluorescence microscopy. This procedure revealed the presence of a monoclonal proliferation of light chain containing plasma cells, and thus led to the diagn...

The B-cell Epitope Interaction Database (BEID; http://datam.i2r.a-star.edu.sg/BEID) is an open-access database describing sequence-structure-function information on immunoglobulin (Ig)-antigen interactions. The current version of the database contains 164 antigens, 126 Ig and 189 Ig-antigen complexes extracted from the Protein Data Bank (PDB). Each entry is manually verified, classified, and an...

Immunoglobulin (Ig) class switching is a process by which B lymphocytes shift from production of IgM to other Ig classes and subclasses via Ig class switch recombination (CSR). Multiple cellular and molecular processes are involved in CSR. Induction of a given IgH germline transcription initiates CSR processes. Ig germline transcription is selectively activated and induced by specific cytokine(...

Mouse immunoglobulin G (Ig G1 and the mixture of Ig G1 and Ig G2) deposited on mica were imaged with an atomic force microscope at room temperature and ambient pressure. At a concentration around 1.0mg/L, the molecules were well dispersed. 2~3 days after sample preparation, both Ig G1 and the mixture could selfassemble into different shapes and further form some types of local-ordered toroidal ...

Lymphocytes from b(5)/b(9) rabbits were stained in suspension with fluorescent antiallotype antibody reagents to selectively label with fluorescent molecules those cells bearing membrane immunoglobulin (Ig) of the b5 or b9 allotype. After staining, the cells were separated by the fluorescence-activated cell sorter into populations markedly enriched in cells bearing b5 or b9 membrane Ig or total...

Immunoglobulin (Ig) antigen receptors are composed of a noncovalently-associated complex of Ig and two other proteins, Ig alpha and Ig beta. The cytoplasmic domain of both of these Ig associated proteins contains a consensus sequence that is shared with the signaling proteins of the T cell and Fc receptor. To test the idea that Ig alpha-Ig beta heterodimers are the signaling components of the I...

Rat spleen lymphocytes were iodinated (125 I) with lactoperoxidase. Quantitative autoradiographic studies on cells fixed immediately after iodination showed 19-24% of intracytoplasmic grains at 3HD and over from the plasma membrane. Normalization of grain density distribution and comparison of resulting curves with the universal curve of grain scatter of 125 I showed that a significant percenta...