BACKGROUND High frequencies of the BRAF V600E mutation have been reported in pleomorphic xanthoastrocytoma (PXA). Recently, a BRAF V600E mutation-specific antibody has been developed and validated. We evaluated the immunohistochemical (IHC) detection of BRAF V600E mutation in PXA by comparing to gold standard molecular analysis and investigating the interobserver variability of the IHC scoring....

Overexpression of HER2 is one of the major causes of breast cancer, and therefore precise diagnosis of its protein expression level is important. However, current methods estimating the HER2-expression level are insufficient due to problem with the lack of quantification. This might result in a gap between diagnostics and therapeutics targeting HER2. Therefore, a new effective diagnostic method...

Although immunohistochemistry (IHC) is a popular imaging technique, the quantitative analysis of IHC images via computer-aided methods is an emerging field that is gaining more and more importance thanks to the new developments in digital high-throughput scanners. In this paper, we discuss the main steps of IHC and review the techniques for computer-aided chromogenic IHC analysis, including met...

By Dean Chang EDITORIAL ASSISTANT To attain the lofty status of All-American would require a combination o f talent, hard work and determination. Going into Ihc Nationals held at Emory University in Atlanta, none of Ihc five women nor their coach of Albany Slate's women's swim team expected thni much from themselves. The Nationals would be a solid learning experience for Ihc groups, and would i...

BACKGROUND Integrative health care (IHC) combines therapies and providers from complementary and conventional health care. Previous studies on IHC have shown power relations between providers but few studies have explored how the interaction develops over time. The objective of this study was to explore the development of IHC collaboration and interaction among participating providers during a ...

This paper aims at presenting a personal experience in teaching the HCI course at the Computer Science Department at the Federal University of Minas Gerais, in Brazil. The course syllabus, how theory and practice are integrated in the course, and the experience to integrate the course work and the Evaluation Competition category available at the Brazilian Symposium of Human Factors in Computing...

Immunohistochemistry (IHC) is an important auxiliary method for pathologists in routine diagnostic work as well as in basic and clinical research including exploration of biomarkers, as IHC allows confirmation of target molecule expressions in the context of microenvironment. Although there has been a considerable progress in automation and standardization of IHC, there are still many things to...

IHC é uma disciplina jovem e há relativamente poucas teorias próprias e de amplo espectro propostas até hoje. A Engenharia Semiótica é uma destas teorias. Organizada de maneira completa há apenas poucos anos, ela foi inicialmente ilustrada com casos da prática profissional de IHC. Este tutorial apresenta seus aportes para a prática científica de IHC, no âmbito da Ciência da Computação. Palavras...

We assessed the validity of immunoblotting, immunohistochemistry (IHC), and immunocytochemistry (ICC) to detect anti-neuronal antibodies in an attempt to establish a diagnostic approach for pediatric autoimmune encephalitis. Both IHC and ICC had higher sensitivity than immunoblotting and could differentiate between antibodies directed towards intracellular and cell surface antigens. There was a...

This study sought to evaluate a new combined gene and protein detection platform in the context of HER2 evaluation in breast and gastric carcinomas. HER2 immunohistochemistry (IHC) and dual color in situ hybridization (Dual ISH) were combined on a single slide. Results were compared with conventional HER2 IHC and fluorescence ISH. Results from the gene and protein assay were reliable and highly...