We studied the ELSA-pS2 immunoradiometric kit (CIS Bio International) for pS2 protein assay in breast cancer cytosols according to classic validation methods. In addition, we studied correlations between pS2, steroid receptors, and cathepsin-D assays. Repeatability (CV = 1.5% to 4.8%) and reproducibility (CV = 1.6% to 4.9%) were good. The results were linearly related to pS2 concentrations betw...

cystic hydatid disease (hydatidosis) is the most serious tape-worm infection prevalent in the cattle and sheep raising area of the world. hydatidosis in man (as an accidental host) caused by infection with the ova containing larval stage of echinococcus spp. in the last decade different techniques have been employed for the serological diagnosis of hydatid disease, such as iha, ifa, elisa, ccle...

Using human liver ferritin as a model, we have developed a two-site immunoradiometric assay. Sample (or standard) is incubated with excess rabbit antiserum and 125I-labeled specific sheep antibodies directed against different antigenic determinants until equilibrium is reached. Then excess sheep anti-rabbit immunoglobulin G serum, covalently linked to magnetizable particles, is added. The radio...

introduction: radiolabeled antibiotics are being used for the specific diagnosis of infection by exploiting their specific binding properties to the bacterial components, thereby making it possible to differentiate infection from sterile lesions. the aim of this work was to prepare and evaluate a freeze-dried kit of ciprofloxacin designed for the labeling with 99mtc. methods: factors like amou...

Various authors (1,2) have tried to ascertain whether CA 125 concentrations in PF samples were significantly higher in patients with endometriosis than in control subjects. So far, these studies have not shown any significant difference in CA 125 values obtained from undiluted PF samples. In our study, using diluted samples in one-step assay, we found that CA 125 concentrations differed between...

BACKGROUND Our renin IRMA overestimated renin in plasmas with high prorenin-to-renin ratios. We suspected that the overestimation of renin was caused less by cross-reactivity of the renin-specific antibody with prorenin than by a conformational change of prorenin into an enzymatically active form during the assay. METHODS Because the inactive form of prorenin converts slowly into an active fo...