the increasing production and use of fossil fuels increase the probability of soil source contamination by oil and petroleum products, and pose a hazard to life. there are over 43,000 chemical contaminated sites in canada and usa, with 2,200 of them classified as high risk. in the us, over $10 billion are spent annually on the cleanup of contaminated sites. the primary objective of this study w...

Meiotic segregation of chromosomes 13 and 14 was studied in the ejaculated spermatozoa of three men carrying a translocation der(13;14)(q10;q10). The spermatozoa of these patients and of a donor with a normal 46,XY karyotype (control) were analysed by two-colour fluorescent in-situ hybridization (FISH) with specific chromosomal painting of chromosomes 13 and 14, by two-colour FISH detecting chr...

Eight cases of 45 XO/46 XY mosaicism are described: four were phenotypic males. Karyotyping should be performed more frequently in short boys as growth hormone treatment and testicular biopsy, to screen for carcinoma in situ, are likely to be beneficial.

In recent years a fascinating evolution of different multicolor fluorescence in situ hybridization (FISH) technologies could be witnessed. The various approaches to cohybridize multiple DNA probes in different colors opened new avenues for FISH-based automated karyotyping or the simultaneous analysis of multiple defined regions within the genome. These developments had a remarkable impact on mi...

the nutritive value of pistachio epicarp (pe) was evaluated by in situ and in vitro techniques. chemical analysis indicated that pe was high in crude protein (11.30%) and low in neutral detergent fiber (26.20%). total phenols, total tannins, condensed tannins and hydrolysable tannins contents in pe were 8.29%, 4.48%, 0.49% and 3.79%, respectively. ruminal dry matter and crude protein degradatio...

The Ts65Dn mouse is a segmentally trisomic model for Down syndrome. Until now, Ts65Dn mice have been identified by the laborious methods of either chromosomal analysis of cultured peripheral lymphocytes or fluorescent in situ hybridization (FISH). We report here a quantitative PCR method for genotyping Ts65Dn mice, as well as a phenotypic description for visually preclassifying mice to be genot...