Background: Cryobiology and in vitro maturation of oocytes are very important tools of reproductive technology. Oocyte plays an important role in fertility. The objective of this research is comparison study the effect of invitro maturation and IVF fertilization of cryopreserved oocyte with fresh oocyte in mice. Materials and Methods: For this purpose, after superovulation was performed oocytes...

Background: Induction of in vitro maturation and development of immature oocyte is one of the methods that has been frequently used recently in artificial reproduction techniques and has drawn attention of many investigators. This method is useful especially in women who are affected by cancer and polycystic ovary syndrome. Despite using many types of in vitro media, an appropriate environment ...

background & aim: considering that antioxidants are known as effective free radicals scavenger, it may be able to improve the in vitro oocyte maturation and the fetal quality. this study was designed to determine the effect of gundelia tournefortii leaves (gte) hydro-alcoholic extract as a source of antioxidant on in vitro oocyte maturation. experimental: germinal vasicle (gv) were recovere...

objective: cryopreservation of ovarian tissue or follicles has been proposed as an alternative method for fertility preservation. although successful vitrification of follicles has been reported in several mammalian species, the survival rate is generally low. the aim of this study was to investigate the effects of fibroblast growth factor (fgf) and epidermal growth factor (egf) on in vitro pre...

Background: Mitochondria are the most aboudent and small essential organelles found in eukaryotic cells. These are semiautonomous organelles for the production of cellular ATP that through its various biochemical pathways. The primary pathway for ATP production is OXPHOS via the electron transfer chain (ETC) which is encoded by nuclear DNA and mtdna genomes. Mitochondria consist of double stran...

Background: Despite great advances achieved in the field of in vitro oocyte maturation (IVM), almost all IVM media are supplemented with undefined component which not only pose the risk of pathogen transmission but also hinders our knowledge of molecular mechanisms of maturation. One of these components is serum that containing various concentration of unknown molecules affecting cellular proce...

Background: Polycystic ovary syndrome (PCOS) is characterized by chronic anovulation and hyperandro genism and affects approximately 5-10% of women of reproductive age. In vitro maturation (IVM) of mammalian oocytes has become an efficient method to produce mature oocytes in order to use in assisted reproductive techniques such as in vitro fertilization (IVF), intra cytoplasmic sperm injection ...

Background & Aim: Oocytes in vitro maturation is an enormously promising technology for the treatment of infertility, yet its clinical application remains limited owing to poor success rates. Therefore this study was designed to determine the effect of Gundelia Tournefortii root hydro alcoholic extract as a source of antioxidant on in vitro oocyte maturation. Experimental: In this stu...

Background & Aim: Oocytes in vitro maturation is an enormously promising technology for the treatment of infertility, yet its clinical application remains limited owing to poor success rates. Therefore this study was designed to determine the effect of Gundelia Tournefortii root hydro alcoholic extract as a source of antioxidant on in vitro oocyte maturation. Experimental: In this stu...