In vitro propagation of Tilia platyphyllos Scop. has been achieved by axillary shoot proliferation and somatic embryogenesis. The influence of tree age, explant source, genotype, and phytohormones on micropropagation of juvenile and mature trees of Tilia platyphyllos has been investigated. Nodal segments and shoot tips were used as initial explants for axillary shoot proliferation. Low concentr...

The aim of this work was to develop protocols for micropropagation of selected Rubus cultivars – ‘Tulameen’ and ‘Black Jewel’ (Rubus idaeus L.), ‘Black Satin’ (Rubus fruticosus L.) and ‘Tayberry’ (Rubus fruticosus x Rubus idaeus). Nodal segments carrying dormant buds were used for in vitro culture establishment. For shoot initiation, MS medium containing 8 g*L phytoagar, 30 g*L sucrose, 1 mg*L ...

A reliable method is described for rapid proliferation of axillary shoots from nodal segment of mature field-grown aromatic and medicinal plant Vitex negundo through in vitro culture. Of different concentrations of N6-benzyladenine (BA) evaluated as supplements to Murahige and Skoog (MS) medium, BA at 1mg/l was effective in enhancing bud break and shoot regeneration. Response of explants was al...

fig trees are threatened by the attack of fig mosaic disease (fmd) on leaves and fruits caused by viruses of several genera. shoot-tip culture is a convenient method for viral sanitation. for this purpose, a reliable protocol for rapid in vitro propagation was developed with shoot-tips of three major tunisian local fig (ficus carica l.) varieties zidi (zdi), soltani (sni), bither abiadh (ba) an...

background and objectives: availability and uptake of mineral by explants are recognized as being important in tissue culture. the limiting factor for maximum mineral uptake and optimum growth is that minerals are not enough available to the explants. there are the inter-relationships between plant growth, mineral uptake, medium water potential, and mineral movement through the medium. plantlet...

RAPD analysis This study aimed to develop an efficient method for micrpropagation of true-to-type date palm plants through direct organogenesis. Nodular cultures were obtained from shoot tips on MS medium plus 2 mg/l 2ip and 1 mg/l NAA. Among combinations of 2ip and 2,4-D added to culture medium for direct shoot buds proliferation, 5 mg/l 2ip alone gave the highest organogenesis frequency. For ...

Vaccinium myrtillus Linn. is an imported fruit tree with high economic value. Currently, in Vietnam there has not been any publication on vitro propagation of the blueberry plant. This study focused V. by nodal culture. The results showed that shoot growth and proliferation were best when nodes cultured WPM medium supplemented 0.5 mg/L zeatin, 30 g/L sucrose, 10 agar, pH 5.8 (shoot height 4.24 ...

lisiantus is introduced among the 10 top cutting flowers in the world. micropropagation can be used in this plant because of some problems in traditional propagation. in this research, the effects of the growth regulators on the in vitro shoot regeneration and rooting of the shoots obtained from nodal explants were investigated. the effects of the different concentrations of bap and kin on shoo...