The emergence of new sequencing technologies has provided fast and cost-efficient strategies for high-resolution mapping of complex genomes. Although these approaches hold great promise to accelerate genome analysis, their application in studying genetic variation in wheat has been hindered by the complexity of its polyploid genome. Here, we applied the next-generation sequencing of a wheat dou...

As small molecules that aid in posttranscriptional silencing, microRNA (miRNA) discovery and characterization have vastly benefited from the recent development and widespread application of next-generation sequencing (NGS) technologies. Several miRNAs were identified through sequencing of constructed small RNA libraries, whereas others were predicted by in silico methods using the recently accu...

The conservation of the linear order (colinearity) of genetic markers along large chromosome segments in wheat and rice is well established, but less is known at the molecular level (microcolinearity). Previous comparative maps between these two species revealed conserved linkage between rice chromosome 2 and wheat chromosome 6. As part of our efforts to identify a locus affecting Grain Protein...

NPF (formerly referred to as low-affinity NRT1) and 'high-affinity' NRT2 nitrate transporter genes are involved in nitrate uptake by the root, and transport and distribution of nitrate within the plant. The NPF gene family consists of 53 members in Arabidopsis thaliana, however only 11 of these have been functionally characterized. Although homologous genes have been identified in genomes of di...

The hsp90-based chaperone machinery is implicated in numerous cellular processes including signal transduction, genomic silencing, and protein degradation. Hop is a component of the animal hsp90 multichaperone complex, whose function is to link the two chaperones, hsp90 and hsp70. Currently there exists little information on a plant Hop homologue. Herein it is reported that a 70-kDa protein in ...

This paper examines common factors that constrain wheat yields: insufficient nutrients (using nitrogen as an example); problems of late planting and poor crop establishment; suboptimal water management; lodging; and weeds. The authors suggest agronomic practices, including tillage practices, rotations, and input management options that can ameliorate important constraints and sustainably improv...

Wheat lines with Fusarium crown rot and nematode resistances arrived in November of 2006 from the laboratory of Dr. Richard Smiley. In this initial collection, we received 69 wheat lines (46 spring and 23 winter) displaying varying levels of resistance to Fusarium crown rot and plant parasitic nematodes including those to root lesion nematodes (RLN). The late arrival of the collection limited o...

We developed EST-PCR markers specific to barley chromosome 2H, for the purpose of effectively tracing alien chromosomes or chromosome parts in the wheat genetic background. The target alien chromosome 2H confers high resistance to pre-harvest sprouting, which is a worldwide natural disaster in wheat. A total of 120 primer pairs were selected by combining the wheat group 2 chromosomes of th...

Previous studies in rodents treated with the pro-carcinogen 1,2-dimethylhydrazine suggested that the consumption of wheat bran protected against DNA damage in the colon and rectum. Based on this information, we evaluated wheat bran as a functional food in the prevention and treatment of colon cancer. We used the aberrant crypt focus assay to evaluate the anticarcinogenic potential of wheat bran...

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