introduction: the antibacterial effects of calcium hydroxide (ch), mineral trioxide aggregate (mta), and calcium enriched mixture (cem) cement against various spp. of microorganisms were assessed in-vitro using agar diffusion test. materials and methods: a base layer of petri plates was made using muller-hinton agar. four cavities were made in agar and filled with freshly mixed materials after ...

Haloarchaea are salt loving microorganisms isolated from saline environments including ancient salt deposits and rock salt. A few haloarchaeal species have been reported to produce bacteriorhodopsin (BR) which works as a light driven proton pump. A haloarchaeal strain Halostagnicola larsenii IBS (MCC 2956) isolated from commercial Indian black salt (IBS) sample was employed for optimization of ...

Four chromogenic media were compared for their ability to detect urinary tract pathogens in 299 urine specimens, of which 175 were found positive, allowing the growth of 279 microorganisms. After 18 to 24 h of incubation, the CPS ID4, CPSE, CPSO (bioMérieux), and UriSelect4 (Bio-Rad) media showed sensitivities of 97.1%, 99.3%, 99.6%, and 99.6%, respectively.

In this report, we describe a simple packaging method which can be used to preserve either normal or salt-agar plates during prolonged incubation. In our search for halophilic microorganisms from Indonesian "Pindang," consisting of boiled and salted fish (S. Iljas and L. J. Ronsivalli. Fishery Ind. Res., in press), it was evident from the literature (2) that prolonged incubation might be necess...

An in vitro experiment was conducted to determine the effect of supplemental non-fiber carbohydrate(NFC) on the disappearance kinetics of cellulose (Ce) by mixed ruminal microorganisms. Non-supplementedor NFC supplemented cellulose (467 mg NFC/g cellulose as sucrose (CeSu) or starch (CeSt) or a 1:1 mixtureof sucrose + starch (CeSuSt)) were incubated for 24, 48, and 96 h at 39°C. After each incu...

Evidence is presented that Zn(2+) and certain other multivalent cations inhibit the uptake and oxidation of substrates by Pseudomonas aeruginosa and by other bacterial cells. Tris(hydroxymethyl)aminomethane augmented this effect. Inhibition by these multivalent cations could be prevented by incubation of cellular suspensions of P. aeruginosa in phosphate buffer. In the case of other microorgani...

The effects of probiotics, yucca extract, monensin and L-cysteine on rumen methanogenesis were determined by in vitro incubation using mixed bovine rumen microorganisms with ground Bermuda grass hay as a substrate. Two forms of probiotics, yucca extract, monensin and L-cysteine were tested during 24 hour anaerobic incubations. Methane and carbon dioxide production and fermentation characteristi...

The occurrence and system of detection of mesophilic and psychrotrophic aerobic sporulating microorganisms (MPAS) in raw cow’s milk were studied. Samples of raw cow’s milk from bulk tanks were collected 21 times from 14 farms during one year. MPAS assessment is based on inactivation of milk sample at the temperature of 80–82°C for 30 minutes followed by incubation in cultivation dishes at 30±1°...

Microbial invasion of egg contents is a cause of embryonic death. To counter infection risks, the embryo is protected physically by the eggshell and chemically by antimicrobial proteins. If microbial pressure drives embryo mortality, then females may have evolved, through natural selection, to adapt their immune investment into eggs. Although frequently hypothesized, this match between immune a...