We wanted to know if incubation time and temperature for many radioimmunoassay methods could be standardized, to decrease assay time and so improve efficiency. Percentage binding was determined for triiodothyronine, thyroxine, digoxin, digitoxin, testosterone, aldosterone, estradiol, and diphenylhydantoin when the reaction mixtures were incubated at 6-8, 22, or 37 degrees C for various periods ...

Transmissible spongiform encephalopathies are neurodegenerative diseases for which prions are the attributed pathogenic agents. A widely accepted theory assumes that prion replication is due to a direct interaction between the pathologic (PrP(Sc)) form and the host-encoded (PrP(C)) conformation, in a kind of autocatalytic process. Here we show that the overall features of the incubation time of...

in this research, the bioinsising impact of two wood decay fungi (pysisporinus vitreus and xylaria longipes) on radial air permeability and moisture diffusion coefficient of spruce wood (picea abies) was investigated. fungal cultivation was performed in an incubator at 25 °c and 85% relative humidity for 15, 30 and 45 days. results showed that the permeability, moisture diffusion and mass loss ...

This article examines the time between product development and market launch, and its relation to the subsequent diffusion of consumer durables. We find that this “incubation time” is long. Further, it is a useful predictor of the shape of the subsequent sales diffusion curve. Using the Bass model as a base, we find that the longer the incubation time, the lower the coefficient of innovation (p...

Crazing is usually a precursor of the brittle fracture of glassy polymers. In this paper, an optical technique is presented to quantify the crazing dominated creep damage that occurs on a stressed transparent glassy polymeric sheet of polymethyl methacrylate (PMMA). The specimens, with various sections, are loaded for a predefined period of time under a constant load at room temperature, and th...

Use of a briefer incubation interval in several steroid radioimmunoassays markedly increases cross reactions with some closely related steroids. The dissociation rates of the various steroid/antibody complexes play a critical role in determining the specificity of the antiserum, and the maximum specificity of an antiserum will be exploited only if it is incubated to equilibrium. The commonly us...

Prion disease incubation time in mice is determined by many factors including PrP expression level, Prnp alleles, genetic background, prion strain and route of inoculation. Sex differences have been described in age of onset for vCJD and in disease duration for both vCJD and sporadic CJD and have also been shown in experimental models. The sex effects reported for mouse incubation times are oft...

to determine the effects of vinasse on physicochemical properties of a calcareous and an acidic soil experiment was factorial and had a complete randomized design and three replications. factorial combinations of five vinasse levels (0, 5, 10, 20 and 40 t/ha) and three exposure times (10, 20 and 30 days) were considered as the treatments of this experiment. the result of experiment showed that ...

abstract- to evaluate effects of different soil moisture conditions namely, phosphorus (p) and zinc (zn) application, on extractable zn and fe, two experiments were conducted in laboratory conditions based on a completely randomized design with a factorial arrangement of treatments with two replications. the first experiment was performed with the following factors; incubation time at four leve...