The occurrence of Toxoplasma antibodies in domestic outpatient cats in the city of São Paulo was evaluated using the indirect immunofluorescence assay. A total of 248 blood samples obtained from male and female cats seen at the Veterinary Teaching Hospital at the University of São Paulo between February 1996 and January 1997 were tested. Of these, 17.7% were positive, with a 64 titer being dete...

From January 2004 to July 2007, 2455 sera were collected from domestic and wild animals living in central Italy and tested by indirect immunofluorescence assay to detect antibodies against Anaplasma phagocytophilum. Considering sera with 1:40 antibody titers as positive, 336 (13.68%) animals scored positive. The percentages of seropositivity were: 46.26% (31/67) in fallow deer, 46.15% (24/52) i...

The immune responses of two S. gallinarum strains, L46 and CN 180, were compared in 15-week-old cockerels. The humoral and cell-mediated immune responses were assayed by means of the indirect haemagglutination test (IHA) and the macrophage migration inhibition test (MIT), respectively. Birds were vaccinated with the two vaccines, respectively, and bled for sera (for IHA) and cells (for MIT) eve...

A new technique, the enzyme-linked immunosorbent assay (ELISA) a@ears to have advantages in some areas over the standard indirect hemagglutination (IHA) test for detecting antibody to Qecific F-l antigen of the plague bacillus, Yersinia pestis. In this presentation the authors compare the results of ELISA and IHA testing and discuss possible ways the former could contribute to solving certain p...

The study objective was to investigate an acute case of Chagas disease in the San Pedro de Shishita community, Pebas District, in the Peruvian Amazon basin, a non-endemic area. Both parents of the index case (acute case) were thoroughly interviewed, a seroepidemiological survey was carried out in the community, parasitological exams were carried out only in relatives of the index case, and tria...

In addition to the indirect and direct costs presented, BRL 917 millions are spent annually with loss of income due to premature death (Table 5). The estimated total amount of potential life years lost was 33,898 years (from the average number of deaths over the years 2012, 2013 and 2014). The maximum age rate was used in a conservative way, to not overestimate the number of days lost due to pr...

The occurrence of bullous pemphigoid (BP) in patients with psoriasis has occasionally been reported. Most previously reported cases attributed the occurrence of BP in psoriasis to photochemotherapy, such as PUVA, UVA and UVB (1–4). We describe here a patient who initially had psoriasis vulgaris and subsequently developed BP after treatment with UVA for psoriasis. Histopathologic, direct immunof...

The MetaCheck (Korr Medical Technologies, Salt Lake City, Utah) is an instrument designed to measure Resting Metabolic Rate (RMR) using indirect calorimetry. Indirect calorimetry is a method of calculating metabolic rate from the measured the amount of oxygen consumed by the body. Using the MetaCheck mouthpiece, the individual being tested breathes in room air and the gas the person breathes ou...

SYNOPSIS The indirect fluorescent antibody technique has been used to titrate patients' antibody against Toxoplasma gondii. Rigorous tests of the immunological validity of the method were made. The reactions of the gamma globulin of human immune serum with Toxoplasma gondii and fluorescent rabbit anti-human gamma globulin were thus shown to be specific. Thereafter the technique could be used wi...

BACKGROUND Antinuclear antibodies (ANAs) in women with recurrent miscarriage have been reported. The presence of moderate to high titers of these antibodies represents an autoimmune condition that can endanger the health of the fetus in pregnant women. OBJECTIVE In this study, we evaluated the prevalence of ANAs in Iranian women with a history of two or more unexplained abortion. MATERIALS ...