A simple, rapid, inexpensive fluorescence polarization assay for the detection of antibodies to Brucella abortus in bulk tank milk samples at the farm level or at dairies with a sensitivity and specificity of 100 and 95.9%, respectively, is described. The assay detects antibodies to B. abortus in 15 min by testing undiluted whey produced by chemical and physical manipulation of milk from bulk t...

Introduction. The development of effective test systems for tuberculosis (TB) diagnosis and the study factors influencing their sensitivity specificity constitute urgent tasks given unstable epidemiological situation with TB in Ukraine world. aimed to determine clinical laboratory features pulmonary that may affect IgG antibody detection human serum by immuno-enzyme system “IB-Chem Anti-Mycobac...

Molecular and metabolic aspects of differentiation of brown adipocytes of the Djungarian hamster (Phodopus sungorus) were studied in primary culture. Expression of uncoupling protein and lipoprotein lipase were investigated by Western and Northern blotting and indirect immuno-fluorescence microscopy. The activity of 5'-deiodinase type II was determined by a radioactive enzyme assay. Activity of...

An assay based on quantitative spectrofluorometry of surface immunofluorescence was applied to the study of reactions between antibody and surface antigens of viable HSV-infected cells in suspension. Fluorescence was expressed in terms of photon counts per second. Both the direct and indirect fluorescent antibody techniques proved acceptable for assay of surface reactions, yielding values of sp...

An antiserum raised against whole cells of Thiobacillus ferrooxidans was allowed to react with a variety of acidophilic and nonacidophilic bacteria in an enzyme-linked immunosorbent assay and an indirect immunofluorescence assay. Both experiments demonstrated that the antiserum was specific at the species level. This preparation was used to evaluate the role of T. ferrooxidans in the microbial ...

A new assay system for chitin has been developed. It comprises the chitin-binding protein ChbB in fusion with a His-tag as well as with a Strep-tag, the latter of which was chemically coupled to horseradish peroxidase. With the resulting complex, minimal quantities of chitin are photometrically detectable. In addition, the assay allows rapid scoring of the activity of chitin-synthases. As a res...