Symptomatic samples of anthracnose-twister disease cultivated onion (Allium cepa) were collected from heavily infected fields at Matale district, Sri Lanka during a severe outbreak that occurred through late July to August in 2020. The pathogen was identified as Colletotrichum siamense based on morphology and combined gene sequence analysis nuclear ribosomal internal transcribed spacers 1 2 wit...

BACKGROUND Accurate identification of sheep nematodes is a critical point in epidemiological studies and monitoring of drug resistance in flocks. However, due to a close morphological similarity between the eggs and larval stages of many of these nematodes, such identification is not a trivial task. There are a number of studies showing that molecular targets in ribosomal DNA (Internal transcri...

Repeated sequences, like the 5.8S rRNA and its flanking regions in C. albicans, can be of particular interest as PCR targets; this is especially true if variations in sequence or length of these regions occur between members of a same genus or species (1). We therefore cloned ribosomal repetitive units of two C. albicans strains and determined the sequence of their respective Internal Transcrib...

The eukaryotic ribosomal DNA cluster consists of multiple copies of three genes, 18S, 5. 8S and 28S rRNAs, separated by multiple copies of two internal transcribed spacers, ITS1 and ITS2. It is an important, frequently used marker in both molecular cytogenetic and molecular phylogenetic studies. Despite this, little is known about intragenomic variations within the copies of eukaryotic ribosoma...

Molecular identification and genotyping of entomopathogenic nematodes (EPNs) are prerequisites for their proper classification, biodiversity studies, and their potential use in biological control programs. In Palestine, although several isolates of EPNs have been collected, phenotypically characterized, and assessed for their tolerance to cold and heat stresses, the molecular identification and...

the genus phaeoacremonium is associated with decline disease of woody plants and with human infections. members of this genus have broad host range and wide geographical distribution. during 2010, ten isolates of phaeoacremonium were recovered from vascular tissues of persimmon (diospyros kaki) trees, showing decline symptoms in shiraz city. anamorphic characteristics such as, conidiophore morp...