objective(s):to determine the fetal discernment in suspected cases of sex linked recessive disease in the first trimester of pregnancy. materials and methods: after collection of 100 chorionic villi samples, the dnas were extracted and baby gender was determined. meanwhile, after increasing the sensitivity, the system was able to detect the sex of each cell which was obtained by biopsy. results...

glucose-6-phosphate dehydrogenase (g6pd) deficiency is the most prevalent enzymopathy in mankind. it has sex-linked in­heritance. this enzyme exists in all cells.  g6pd deficiency increases the sensitivity of red blood cells to oxidative dam­age. g6pd deficiency was discovered in 1950 when some people suffered hemolytic anemia as a result of taking antimalar­ial drugs (primaquin). most people w...

results the optimal conditions were considered to perform elisa. comparison between results obtained from optimized elisa kit and commercial elisa kit showed a good agreement. background haemophilus influenzae type b (hib) infection has high morbidity and mortality rate, especially in children under 5 years of age. enzyme-linked immunosorbent assay (elisa) technique is the most used method to d...

background: diarrhea, caused by enteroaggregative escherichia coli (eaec), is an important cause of illness and death. numerous virulent factors have been described in eaec. however, their prevalence was highly variable among eaecs of distinct geographic locations. studies have shown that dispersin (antiaggregation protein, aap) is one of the important and abundant virulent factors in eaec. in ...

introduction: periodontitis is a multi-factorial inflammatory disease of periodontium. a precise mechanism in which smoking affects periodontium is not accurately understood. this study was planned to survey salivary osteocalcin (oc) level among smokers with chronic periodontitis and its comparison to non-smoker patients which may help achieve a better understanding of mechanisms being involved...

background: pan-igg specific monoclonal antibodies (mabs) are essential tools for assessment of humoral immunity, immune deficiency and gammopathy. in this study, four hybridoma clones producing mabs with different specificities for human igg isotypes were established. methods: splenocytes from balb/c mice immunized with fc fractions of human igg were fused with sp2/0 myeloma cells. hybridoma c...

universal vaccination of neonates and children against diphtheria, tetanus and pertussis has had a tremendous impact on the control of these infectious diseases worldwide. immunization by the triple diphtheria, tetanus and whole cell pertussis vaccine (dtwp) has been applied in iran for almost 50 years. periodic assessment of immunogenicity of this vaccine is an important aspect of successful m...

oral-facial-digital syndrome as heterogeneous developmental conditions is characterized by abnormalities in the oral cavity, facial features and digits. furthermore, central nervous system (cns) abnormalities can also be part of this developmental disorder. at least 13 forms of ofds based on their pattern of signs and symptoms have been identified so far.  type 1 which is now considered to be a...

background and aims: the causal agents of viral lettuce big vein disease are two viruse, lettuce big vein associated virus (varicosavirus) and mirafiori lettuce virus (ophiovirus). these viruses have coat proteins of similar size but have different morphologies and serologically unrelated.the purpose of this study was to distinguish and detect lbvav and milv in lettuce fields in tehran province...