We compared two techniques for separating and evaluating serum creatine kinase isoenzymes--fluorometric agarose electrophoresis and Sephadex chromatography--in 50 patients, 25 of whom had confirmed acute myocardial infarction. In every case isoenzyme MB (heart isoenzyme) was detected with equal sensitivity by either procedure. Evidently, only the presence or absence of MB is clinically signific...

We describe a simple technique for reacting CK isoenzymes with a substrate in gelatin to make them visible after they have been separated by cellulose acetate electrophoresis. The electrophoresed strip requires only 5 min of contact with the substrate in gelatin and about 12 min of drying in an oven at 55 degrees C. The developed isoenzyme bands are discrete, intense, and rectangular in shape. ...

The hypothesis that hepatitis B infection is etiologically related to hepatoma has been investigated by studying the interrelationships between hepatitis B surface antigen (HBsAg, Australia antigen) and the fast-moving 5'-nucleotide phosphodiesterase Band V isoenzyme (5'-NPDase-V). Sera from 58 patients with viral hepatitis were tested for 5'-NPDase-V and HBsAg. The isoenzyme was found in 34 of...

A new isoenzyme of alkaline phosphatase (EC 3.1.3.1) has been reported to occur in sera from patients with lymphoproliferative diseases. This enzyme is character ized by an inability to hydrolyze cysteamine S-phosphate. We find that the 5,5 -dithobis(2-nitrobenzoic acid)-coupled assay method for cysteamine S-phosphate hydrolysis is not suitable for serum, and we were unable to confirm the exist...

Serum of cancer patients often contains high activities of a homoarginine-sensitive isoenzyme of alkaline phosphatase with high electrophoretic mobility, which is present in relatively low activities in sera from most normal persons and persons with benign disease. In earlier studies of this isoenzyme, in a semi-quantitative assay, electrophoresis was used in the separation step. This report de...

1. The isoenzyme pattern of phosphohexose isomerase on starch-gel electrophoresis was found to be significantly and consistently different in the muscles of the Swiss white and 129 ReJ mice. This provided a good marker for identifying donor and host muscle. 2. The isoenzyme pattern does not appear to be due to different proportions of type 1 and 2 fibres or to fibre type grouping as is found in...

We examined the stability of human lactate dehydrogenase (EC 1.1.1.27) isoenzyme 5--purified to a specific activity of about 400 kU/g--when lyophilized in a buffered, stabilized matrix of bovine albumin. This isoenzyme was prepared with a final activity of about 500 U/L and stored at -20, 4, 20, 37, and 56 degrees C for as long as six months. This isoenzyme decayed with approximate first-order ...

We used ion-exchange column chromatography and electrophoresis on polyacrylamide gel to compare the acid phosphatase isoenzymes of prostate and leukocytes. The major isoenzyme of the prostate is band 2A; only a trace of band 2B was observed. However, the major isoenzymes of leukocytes are band 4 and band 2B, and only a small amount of band 2A was observed. The three isoenzymes isolated from leu...

The structural relationships among human alkaline phosphatase isoenzymes from placenta, bone, kidney, liver and intestine were investigated by using three criteria. 1. Immunochemical characterization by using monospecific antisera prepared against either the placental isoenzyme or the liver isoenzyme distinguishes two antigenic groups: bone, kidney and liver isoenzymes cross-react with anti-(li...

during the period from 1996 to 1997, 45 isolates of leishmania were recovered frompatients suspected of cutaneous leishmaniasis (cl) and one from a gerbil. these specimens werereceived from various parts of iran. isoenzyme profiles of these isolates were compared with those ofreference strains of l.tropica, l.major and l.infantum using cellulose acetate electrophoresis (cae) and10 enzyme system...