Polymerase chain reaction is the most widely used method for in vitro DNA amplification. However, it requires thermocycling to separate two DNA strands. In vivo, DNA is replicated by DNA polymerases with various accessory proteins, including a DNA helicase that acts to separate duplex DNA. We have devised a new in vitro isothermal DNA amplification method by mimicking this in vivo mechanism. He...

This work aims at optimizing sludge pretreatment by non-isothermal sonication, varying frequency, US power (PUS) and intensity (IUS varied through probe size), as well as hydrostatic pressure and operation mode (continuous vs. sequential - or pulsed - process). Under non isothermal sonication sludge solubilization results from both ultrasound disintegration and thermal hydrolysis which are conv...

The thermal decomposition kinetics of melaminium bis(trichloroacetate) dihydrate (MTCA) has been studied by thermogravimetry and derivative thermogravimetry techniques using non-isothermal experiments at three different heating rates 10, 15, and 20 ◦C min−1. Non-isothermal studies of MTCA revealed that the decomposition occurs in three stages involving dehydration and decomposition. The apparen...

In this study, the use of isothermal microcalorimetry (IMC) for differentiation between methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) and MIC determination was evaluated. It was possible to differentiate between MRSA and MSSA within 4 h, whereas the standard method required 24 h. The MICs of cefoxitin were successfully determined for MRSA and MS...

Abstract: The difference between quantum isoenergetic process and quantum isothermal process comes from the violation of the law of equipartition of energy in the quantum regime. To reveal an important physical meaning of this fact, here we study a special type of quantum heat engine consisting of three processes: isoenergetic, isothermal and adiabatic processes. Therefore, this engine works be...

We investigate the dynamic thermal performance of a molten-salt packed-bed thermal energy storage (TES) system using capsules filled with high-temperature phase change material (PCM), which is identified as a promising low-cost TES system for concentrating solar power (CSP) plants. A transient twodimensional dispersion-concentric (D-C) model is modified to account for the phase change process w...

thermodynamic study on the interaction of β-cd with poly ethylene oxide and poly acrylic acid was performed by isothermal titration calorimetry at 298k. when β-cd is added to the interpolymer complex, competition is created between host-guest and hydrogen bond. enthalpy of interaction between the β-cd and interpolymer complex was calculated using the extended solvation theory. p=1 shows that wi...

This paper describes the application of the Radial Functions Method to model and simulate polymer processing. The coupled system of energy and motion equations for laminar two-dimensional flows in steady state of incompressible non-Newtonian fluids is considered. The results delivered by RFM are compared with numerical results of other methods, and with analytical solutions based on simplified ...

Recently, the grain boundary−intragranular nucleation of ferrite has received great attention seeking for the improvement of the toughness in the steel, specially when conventional austenite grain refinement techniques are not enough. This paper deals with some relevant aspects about the competitive process of allotriomorphic and idiomorphic ferrite transformations in a medium carbon vanadium-t...