With a sharp increase in the cases of multi-drug resistant (MDR) bacteria all over the world, there is a huge demand to develop a new generation of antibiotic agents to fight them. As an alternative to the traditional drug discovery route, we have designed an effective antibacterial agent by modifying an existing commercial antibiotic, kanamycin, conjugated on the surface of gold nanoparticles ...

Introduction: Monitoring and controlling water resources and using health risk assessment approaches for water pollutants are essential for health promotion programs. This study aims to determine the water quality status and its spatiotemporal variation across the Kan River Basin, explore the interrelationship between surface and groundwater quality indices, and assess the nitrate health risk i...

A strain of Escherichia coli with the fol gene deleted and a kan gene inserted in its place was created for use in cloning and isolation of mutant dihydrofolate reductase. Southern blot analysis and dihydrofolate reductase enzyme assays confirmed the delta fol::kan genotype. A thyA mutation accompanied the fol deletion and is required for survival of a dihydrofolate reductase-deficient strain.

The categorical concept of Kan extensions form a more general notion of both limits and adjoints. The general definition of Kan extensions is given and motivated by several concrete examples. After providing the necessary background on some basic categorical objects and theorems, the relationship between Kan extensions, limits, and adjoints is expressed through two theorems from [3]. 1. Some Pr...

Bacterial strains and growth conditions All strains used in this study are derivatives of the E.coli K-12 strains W3110 (Hayashi et al. 2006) or MC4100 (Casadaban 1976). While curli production is similar in the two strains, only W3110 is motile, but MC4100 is non-motile as it carries an insertion of a T between nucleotide 123 and 124 in flhD (an allele previously termed flbB5301; Fig. S1). Muta...

Molecular diagnostic assays, with their ability to rapidly detect resistance-associated mutations in bacterial genes, are promising technologies to control the spread of drug-resistant tuberculosis (DR-TB). Sequencing assays provide detailed information for specific gene regions and can help diagnostic assay developers prioritize mutations for inclusion in their assays. We performed pyrosequenc...

Antibiotics are widely used and their abuse has caused ecological hazard. Recently, pollution from pharmaceuticals and personal care products (PPCPs) has aroused great concern among governments and researchers. In order to elucidate the correlations among molecular structure, transmembrane distribution and toxicological effects of different kinds of antibiotics, zebrafish (Danio rerio) embryos ...

F plasmid derivatives carrying kan insertion mutations in the transfer region genes traR, trbJ, and trbH were constructed. Standard tests indicated that these loci are not essential for F pilus production or F transfer among Escherichia coli K-12 hosts. Among the traR and trbH mutants tested, the orientation of the kan cassette had no effect on the mutant phenotype. In each case, there was no s...

The streptomycin (SM) gene cluster was investigated for its distribution in streptomycetes by Southern hybridization using nick-translated DNA probes, which were isolated from the SM-6-phosphotransferase (SPH) and amidinotransferase (ADT) regions of the SM gene cluster of Streptomyces griseus SS-1198. Bgl II-digested genomic DNAs from SM-producing strains of S. griseus yielded the same size fra...

This paper involves categories and computer science. Gröbner basis theory is a branch of computer algebra which has been usefully applied to a wide range of problems. Kan extensions are a key concept of category theory capable of expressing most algebraic structures. The paper combines the two, using Gröbner basis techniques to compute certain kinds of Kan extension.