Carbapenem resistance in Gram-negative bacteria is a worldwide increasing and one of the most disturbing problems, given these antibiotics are drugs of choice in the treatment of infections caused by extended-spectrum-beta-lactamase producing strains. In this study the antibiotic susceptibility of metallo-beta-lactamase-positive and negative Klebsiella pneumoniae strains isolated from intensive...

This study shows for the first time the mechanism of carbapenem resistance of a Klebsiella pneumoniae clinical isolate TJ8 recovered from Tianjin Medical University General Hospital ,China. The modified Hodge test and EDTA synergy test were performed for the screening of carbapenemases and metallo-β-lactamases (MBLs), respectively. Polymerase chain reactions and DNA sequencing confirmed that th...

PURPOSE This study was undertaken to analyze the extended spectrum of β lactamase (ESBL), metallo β lactamase (MBL) & AmpC production in Pseudomonas aeruginosa in various clinical samples. MATERIALS & METHODS One hundred four non repetitive clinical specimens were inoculated onto nutrient agar, blood agar and incubated at 37(o)C overnight. The colonies were tested for oxidase test and other b...

OBJECTIVES To report the identification of the metallo-beta-lactamase (MBL) variant VIM-19 in a Klebsiella pneumoniae clinical strain co-producing KPC-2 carbapenemase, CMY-2 cephalosporinase and CTX-M-15 extended-spectrum beta-lactamase. METHODS MICs were determined by agar dilution. Phenotypic tests were performed to detect carbapenemase production. PCR and nucleotide sequencing were used fo...

Pseudomonas aeruginosa is one of the commonest multi drug resistant bacteria causing nosocomial infection and Metallo-Beta-Lactamase production is one of the important reasons. The following study was conducted to determine incidence of MBL producing P.aeruginosa. and co existence of AmpC and ESBL production. Screening for MBL was done using imipenem disk and all the isolates found resistant we...

Background. Recent reports suggest that metallo-β-lactamase (MBL)-producing Enterobacter cloacae increases as a pathogen of healthcare-associated infections in Japan. Bacteremia caused by MBL-producing E. cloacae is a major concern due to the resistance to most β-lactams including carbapenems. However, little is known about MBL-producing E. cloacae bacteremia in patients with cancer. The aim of...

BACKGROUND The modified Hodge test (MHT) was designed to detect carbapenemase-producing Enterobacteriaceae (CPE). This study evaluated variables to improve the performance of MHT. METHODS Carbapenem-resistant Enterobacteriaceae isolated from November 2010 to March 2013 at the Asan Medical Center, were evaluated, including 33 metallo-β-lactamase (MBL) producers and 103 non-CPEs. MHT was perfor...

INTRODUCTION The emergence and rapid spread of blaIMP and blaVIM metallo-beta-lactamase (MBL) producing Gram-negative bacteria causing nosocomial infections are of concern worldwide due to limited treatment options. METHODOLOGY A total of 179 nonreplicate, consecutive, carbapenem resistant Pseudomonas aeruginosa (61), Acinetobacter baumannii (116), Acinetobacter lwoffii (1) and Pseudomonas st...

The development of broad-spectrum metallo-beta-lactamase (MBL) inhibitors is challenging due to structural diversity and differences in metal utilisation by these enzymes. Analysis of structural data, followed by non-denturing mass spectrometric analyses, identified thiols proposed to inhibit representative MBLs from all three sub-classes: B1, B2 and B3. Solution analyses led to the identificat...

MBL production has been increasingly reported among Pseudomonas aeruginosa, which is an important nosocomial multidrug resistant pathogen. 2,3 Metallo beta lactamase (MBL) belongs to Ambler class B enzymes. With the help of divalent cation zinc as cofactor for enzymatic activity, they hydrolyze carbapenem as-well-as other blactam antibiotics. The substrate spectrum of this enzyme is quite broad...