In the preceding paper the preparation and characterization of antiserum to purified D-lactate are described. In this paper the effects of the antibody on D-lactate dehydrogenase activity and D-lactate-dependent active transport in native Escherichia coli ML 308-225 membrane vesicles and ML 308-225dld-3 vesicles reconstituted with D-lactate dehydrogenase are described. The results demonstrate t...

Kinetic methods are described for determination of total, heat-stable, and urea-stable lactate dehydrogenase (EC 1.1.1.27) activity on the centrifugal analyzer. In the urea-stable method we present, 2.6 molar urea is used to differentiate lactate dehydrogenase originatingfrom heart and liver.This urea concentration, greater than that used by most other investigators, better differentiates betwe...

The existence of a lactate dehydrogenase-immunoglobulin A kappa complex was demonstrated as a marked increase of lactate dehydrogenase activity at the lactate dehydrogenase-3 band in the agar-agarose gel-electrophoresis pattern of sera from seven patients from an unselected group of 21 800 patients. The complex was isolated, in an almost pure form, by gel filtration and affinity chromatography,...

Heart tissue injury may release cardiac enzymes into the circulation and elevate serum enzyme levels (LaDue, Wroblewski, and Karmen, 1954). Many enzymes become raised, but three enzymesaspartate aminotransferase (EC 2.6.1.1), creatine kinase (EC 2.7.3.2), and lactate dehydrogenase (EC 1.1.1.27)-have proved of particular diagnostic value. In addition, determination of lactate dehydrogenase isoen...

Lactate dehydrogenase isozyme 5 from rabbit skeletal muscle is activated by citrate, cis-aconitate, isocitrate, alpha-ketoglutarate, succinate, fumarate, malate, aspartate, and glutamate. In the presence of these activators the shape of the pyruvate saturation curve is changed from sigmoid to hyperbolic. Lactate dehydrogenase isozyme 1 from rabbit heart gives a hyperbolic pyruvate saturation cu...

Background: Toxoplasma gondii is an intracellular protozoan with worldwide distribution capable of infecting all endothermic vertebrates. Latent toxoplasmosis in man and animals is considered as clinically asymptomatic. The aim of this Study is evaluation of the effects of Toxoplasma gondii on Biochemical Parameters of Reproductive Function in Male Rats. Materials and Methods: A total of 56 mal...

The enzymes phosphohexose isomerase, lactate dehydrogenase, hydroxybutyrate dehydrogenase, isocitric dehydrogenase, glutamate dehydrogenase, and sorbital dehydrogenase were assayed in cases of known carcinoma of the cervix and of carcinoma in situ. Phosphohexose isomerase, lactate dehydrogenase, and isocitric dehydrogenase were also assayed in a number of normal women and in those with benign l...

the present study was aimed to evaluate the therapeutic potential of pimpinella tirupatiensis (pt) by assaying the activities of selective mitochondrial enzymes in streptozotocin induced diabetic rats. diabetic rats showed a significant (p < 0.01) reduction in the activities of oxidative enzymes succinate dehydrogenase (sdh), malate dehydrogenase (mdh), glutamate dehydrogenase (gdh) and isocitr...

1. Human foetal skeletal muscles involved in support and in periodic contractility were studied for their content of total extractable lactate dehydrogenase, glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase activities as well as for the relative distribution of lactate dehydrogenase isoenzymes. 2. During foetal development a linear steady increase in total lactate dehydrog...