The rat fatty acid synthase (FAS) is active only as a dimer, although the eight component functions are contained in a single polypeptide chain. Using mRNA from lactating rat mammary glands a cDNA expression library was established. With the overlapping immunologically positive clones we have an 8.9kb cDNA sequence for rat FAS. In the 3'-nontranslated region of the rat FAS cDNA we find a protot...

Fatty acid synthesis in the mammary gland of lactating rats in vivo was 5-fold higher than in the liver. Starvation decreased fatty acid synthesis in the gland 50-fold, whereas refeeding for 2h completely reversed this change. The plasma insulin concentration decreased 2-fold in starvation and was restored to the fed-rat value on refeeding. Glucagon and prolactin concentrations did not always c...

of the bile acid pool in the lactating rat-hydroxylase expression and size α Increased cholesterol 7 You might find this additional info useful... published 12 times a year (monthly) by the American Physiological Society, 9650 Rockville Pike, Bethesda MD involving normal or abnormal function of the gastrointestinal tract, hepatobiliary system, and pancreas. It is publishes original articles per...

The transcellular Ca2+fluxes required for milk production must be rigorously regulated to maintain the low cytosolic Ca2+concentrations critical to cell function. Ca2+-ATPases play a critical role in the maintenance of this cellular Ca2+ homeostasis. Using RT-PCR and sequencing, we identified six Ca2+pumps in lactating mammary tissue. Three plasma membrane Ca2+-ATPases (PMCAs) were found (PMCA1...

Background RFamide-related peptide-3 (RFRP-3) inhibits gonadotropin releasing hormone (GnRH) and luteinizing hormone (LH) secretion in rats. This study evaluates the effects of litter size and suckling intensity on RFRP mRNA expression in the dorsomedial hypothalamic nucleus (DMH) of rats. MaterialsAndMethods A total of 32 pregnant and 4 non-lactating ovariectomized (control group) Sprague-Dawl...