We present a new technique for measuring velocity gradients for laminar shear flow, using dynamic light scattering in the strongly multiple-scattering regime. We derive temporal autocorrelation functions for multiply scattered light, taking into account particle displacements arising from deterministic shear flow and random Brownian motion. The laminar shear flow and Brownian motion are charact...

We investigated the effect of hemodynamic shear forces on the expression of adhesive molecules, E-selectin, and intercellular adhesion molecule-1 (ICAM-1) on human umbilical vein endothelial cells (HUVEC) exposed to laminar (8 dynes/cm2) or turbulent shear stress (8.6 dynes/cm2 average), or to a static condition. Laminar flow induced a significant time-dependent increase in the surface expressi...

The unsteady, incompressible boundary layer flow caused by an impulsively stretching surface under the influence of a transverse magnetic field is investigated. The partial differential equations governing the laminar flow, under boundary-layer approximations, are non-dimensionalised using similarity transformations and then solved numerically using an efficient, implicit finite-difference sche...

OBJECTIVE The liver X receptors (LXRs) regulate a set of genes involved in lipid metabolism and reverse cholesterol transport. We investigated the mechanism by which shear stress regulates LXR in vascular endothelial cells (ECs). METHODS AND RESULTS Western blot showed that the protein level of LXRalpha and its target ABCA1 in the mouse thoracic aorta was higher than that in the aortic arch. ...

Arterial hemodynamic shear stress and blood vessel stiffening both significantly influence the arterial endothelial cell (EC) phenotype and atherosclerosis progression, and both have been shown to signal through cell-matrix adhesions. However, the cooperative effects of fluid shear stress and matrix stiffness on ECs remain unknown. To investigate these cooperative effects, we cultured bovine ao...

Endothelial cells respond to fluid shear stress through mechanotransduction responses that affect their cytoskeleton and cell-cell contacts. Here, endothelial cells were grown as monolayers on arrays of microposts and exposed to laminar or disturbed flow to examine the relationship among traction forces, intercellular forces, and cell-cell junctions. Cells under laminar flow had traction forces...

The effect of inlet conditions on the mixing of a passive scalar was investigated in a planar shear layer with inlet boundary layers that were laminar, tripped and naturally turbulent–transitional. Planar laser-induced fluorescence measurements of acetone were used to directly evaluate the shear layer structure, and were processed to determine probability density functions ~PDFs! of the mixture...

The prediction of the entropy generation rate in laminar shear layers is treated as steady, even in the presence of high levels of free stream turbulence. Here we highlight the deficiencies of this approach by quantifying the magnitude of entropy generation rate fluctuations in the laminar boundary layer subjected to free stream turbulence. We find fluctuation levels in excess of 100% in the ne...

While it is universally accepted that plankton continually experience a dynamic fluid environment, their sensitivity to the features of the surrounding flow field at the relevant length and time scales of the organism is poorly characterized. The present study uses bioluminescence as a tool to understand how the red tide dinoflagellate Lingulodinium polyedrum (5 Gonyaulax polyedra) responds to ...

The equine laminar junction plays a vital role in transferring the forces of weight-bearing between the epidermal hoof wall and the bone of the third phalanx, but the way in which it performs this function is poorly understood. Using samples from sites varying proximodistally and circumferentially around the hoof, the stress/strain behaviour of this tissue was characterised in three directions:...