Desmosomes are not formed in epithelial cell cultures growing in media with low (less than or equal to 0.1 mM) concentrations of Ca2+ (LCM) but appear rapidly upon shift to media of normal calcium concentrations (NCM). Previous authors using immunolocalization of desmoplakin, a marker protein for the desmosomal plaque, in LCM-grown cells have interpreted positively stained, dense, cytoplasmic a...

Laser capture microdissection (LCM) is a technique by which individual cells can be harvested from tissue sections while they are viewed under the microscope, by tacking selected cells to an adhesive film with a laser beam. Harvested cells can provide DNA, RNA, and protein for the profiling of genomic characteristics, gene expression, and protein spectra from individual cell types. We have opti...

Laser capture microdissection (LCM) allows microscopic procurement of specific cell types from tissue sections that can then be used for gene expression analysis. In conventional LCM, frozen tissues stained with hematoxylin are normally used to the molecular analysis. Recent studies suggested that it is possible to carry out gene expression analysis of formaldehyde-fixated paraffin embedded (FF...

Throughout this note, let N denote the set of nonnegative integers. Define N∗ := N \ {0}. There are lots of known results about the least common multiple of a sequence of positive integers. The most renowned is nothing else than an equivalent of the prime number theory; it says that log lcm(1, 2, ..., n) ∼ n as n approaches infinity (see, for instance [6]), where lcm(1, 2, · · · , n) means the ...

‘Laser microdissection: a day for answers conference,’ organized by EuroSciCon (Hertfordshire, UK), hosted a large group of scientists from across Europe. Laser-capture microdissection (LCM) is a state-of-the-art technique that has made a large impact amongst the scientific community in recent times. Despite the usefulness of this technique, LCM is still relatively underused and, thus, the main...

Received March 13, 2007 Abstract For any positive integer n, the Smarandache LCM dual function SL∗(n) is defined as the greatest positive integer k such that [1, 2, · · · , k] divides n. The main purpose of this paper is using the elementary method to study the calculating problem of a Dirichlet series involving the Smarandache LCM dual function SL∗(n) and the mean value distribution property o...

This study used finite element (FE) analysis with the load-controlled method (LCM) and the displacement-controlled method (DCM) to examine motion differences at the implant level and adjacent levels between fusion and non-fusion implants. A validated three-dimensional intact (INT) L1-L5 FE model was used. At the L3-L4 level, the INT model was modified to surgery models, including the artificial...

Four ruminally cannulated buffalo bulls (300 kg) were fed mottgrass diets using a 4 4 Latinsquare design. Treatments consisted of mottgrass fertilized with 0 or 110.4 kg N/ha and harvested at 40 (ECM) and 60 (LCM) days. Fertilization increased the concentrations of crude protein (CP) and fibre contents. The CP contents of the mottgrass decreased and fibre contents increased with advancing matur...

Tissue injury (chronic disease) associated with persistent LCM infection is apparently caused by the host immune response to the virus. Employing parabiosis or cell transfer from hyperimmune donors to isologous virus carriers, the tissue injury of chronic disease could be initiated and/or intensified. Furthermore, the transfer of anti-LCM antibody to SWR/J carrier mice results in acute necrotiz...

Due to the complexity of Alzheimer's disease, multiple cellular types need to be targeted simultaneously in order for a given therapy to demonstrate any major effectiveness. Ultrasound-sensitive coated microbubbles (in a targeted lipid nanoemulsion) are available. Versatile small molecule drug(s) targeting multiple pathways of Alzheimer's disease pathogenesis are known. By incorporating such dr...