Background: Physiological effects of obesity are increasingly common in women of reproductive age and are associated with infertility. Obese women have dysfunction in hypothalamic-pituitary-ovarian axis, menstrual cycle and are up to three times more likely to have decreased oligo-/anovulation. The purpose of present study was to evaluate the high fat diet on IVM and IVF outcomes in mouse. Mate...

Oocytes retrieval, in vitro maturation (IVM) and fertilization (IVF) efficiency are inevitable steps towards in vitro production of embryos. In the present study, these parameters were investigated in the ovaries of prepubertal (n = 31) and pubertal (n = 61) black Bengal goats obtained from a slaughterhouse. Nuclear maturation was evaluated upon aspiration and following IVM in TCM-199 (Earle's ...

Since the pig is not only an important farm animal, but also a model animal for biomedical applications, the development of reproductive technologies in this species has been very important. In vitro oocyte maturation and fertilization (IVM-IVF) are basic techniques for a number of oocyte- or embryo-related technologies. The practical aspects for pig oocyte IVM-IVF have been reviewed, while the...

The inferior oocytes (IOs), which are not suitable for embryo development, occupy roughly one-third or more of the collected immature bovine oocytes. The IOs are usually discarded from the in vitro bovine embryo production process. Improving the quality of the inferior oocytes (IOs) and make them available in in vitro embryo production would have important biological, as well as commercial, val...

background: the presence of rennin-angiotensin components in mammalian ovaries and their involvement in ovarian physiology have been established. in the present study, effects of angiotensin ii (ang ii) on sodium-potassium adenosine triphosphatase (na+/k+/atpase) expression and development of sheep embryos was evaluated. methods: the abattoir-derived cumulus oocyte complexes (coc) were randomly...

OBJECTIVE We compared the assisted reproductive technology (ART) outcomes among infertile women with polycystic ovary syndrome (PCOS) treated with IVM, conventional IVF, GnRH agonist, and GnRH antagonist cycles. METHODS The prospective study included a total of 67 cycles in 61 infertile women with PCOS. The women with PCOS were randomized into three IVF protocols: IVM/IVF with FSH and hCG pri...

introduction: in this study in vitro maturation, resumption of meiosis and development of immature mouse oocytes in the presence of cysteamine was evaluated. meterial and methods: immature oocytes recovered from nmri mouse strain (4-6 weeks) were categorized in three experimental and one control group. the control included 345 immature oocytes which were matured in mem medium contain 5% fcs. in...

The study was conducted to compare differences in vitro maturation media and their effect on subsequent embryo production. procedures were approved by the agricultural research council animal ethics committee (Ref no: APAEC 2020/05) Tshwane university of technology AREC 2021/08/004). Follicular fluid containing a suspension oocytes retrieved from ovaries through aspiration slicing techniques. g...

Mesenchymal stem cells (MSCs) secrete a variety of cytokines and growth factors in addition to self-renewal and multiple forms of differentiation. Some of these secreted bioactive factors could improve meiotic maturation in vitro and subsequent embryo developmental potential. The aim of the present study was to determine whether in vitro maturation (IVM) of mouse oocyte with or without cumulus ...

BACKGROUND The presence of rennin-angiotensin components in mammalian ovaries and their involvement in ovarian physiology have been established. In the present study, effects of angiotensin II (Ang II) on sodium-potassium adenosine triphosphatase (Na(+)/K(+)/ATPase) expression and development of sheep embryos was evaluated. METHODS The abattoir-derived Cumulus Oocyte Complexes (COC) were rand...