Listeria monocytogenes is a species of foodborne pathogen often related to foods, such as poultry, ready-to-eat products, fruits, and vegetables. The culture method is a standard procedure for the detection of bacteria in food products. The real-time quantitative PCR (qPCR) technique can be used for the quantification of foodborne pathogens. The current research was aimed to assess...

Molecular techniques based on real-time polymerase chain reaction (qPCR) allow the detection and quantification of DNA but are unable to distinguish between signals from dead or live cells. Because of the lack of simple techniques to differentiate between viable and nonviable cells, the aim of this study was to optimize and evaluate a straightforward test based on propidium monoazide (PMA) dye ...

The sensitive and specific analysis of microRNAs (miRNAs) without using a thermal cycler instrument is significant and would greatly facilitate biological research and disease diagnostics. Although exponential amplification reaction (EXPAR) is the most attractive strategy for the isothermal analysis of miRNAs, its intrinsic limitations of detection efficiency and inevitable non-specific amplifi...

Asteroid exploration has been attracting more attention in recent years. Nevertheless, we have just visited tens of asteroids while discovered than one million bodies. As our current observation and knowledge should be biased, it is essential to explore multiple directly better understand the remains planetary building materials. One mission design solutions utilizing asteroid flyby cycler traj...

Equine influenza is a cause of epizootic respiratory disease of the equine. The detection of equine influenza virus using real-time Light Cycler reverse transcription (RT)-PCR technology was evaluated over two influenza seasons with the analysis of 171 samples submitted for viral respiratory disease. Increased sensitivity was found in overall viral detection with this system compared to Directi...

Scientific conferences fulfil many roles, but one of the most mportant ones is that they help shape the direction in which a scintific discipline grows by promoting person-to-person exchanges f information, ideas and constructive criticisms between scientists rom different backgrounds. This interaction also helps to idenify areas of controversy and promotes efforts to address and, it is oped, r...

Tracing garbage collection (GC) identifies dead objects by scanning the object-graph from the root set references. It is proven to be highly efficient and widely used in commercial managed runtime systems for Java, C# and scripting languages. However, tracing GC cannot identify the dead objects before finishing object-graph traversal. Hence the application cannot reuse the space of the dead obj...

To facilitate the diagnosis of enterotoxigenic Escherichia coli (ETEC) infections in humans, we developed and evaluated real-time fluorescence PCR assays for the Roche LightCycler (LC) against the enterotoxin genes commonly present in strains associated with human illness. Separate LC-PCR assays with identical cycling conditions were designed for the type I heat-labile enterotoxin (LT I) and th...

Diatoms are unicellular algae, which due to their importance for the global primary production and their cellular and genetic complexity, became popular subjects of physiological and molecular biological research in the recent years. The increasing genomic information gathered on diatoms since the last decade promotes diverse analyses of their steady state RNA levels, which are commonly perform...

Quantitative real-time PCR (qPCR) assays that target the human-associated HF183 bacterial cluster within members of the genus Bacteroides are among the most widely used methods for the characterization of human fecal pollution in ambient surface waters. In this study, we show that a current TaqMan HF183 qPCR assay (HF183/BFDrev) routinely forms nonspecific amplification products and introduce a...