The triglyceride lipase gene subfamily plays a central role in lipid and lipoprotein metabolism. There are three members of this subfamily: lipoprotein lipase, hepatic lipase, and endothelial lipase. Although these lipases are implicated in the pathophysiology of hyperlipidemia and atherosclerosis, their structures have not been fully solved. In the current study, we established homology models...

Lipases (E.C. 3.1.1.3) can be broadly defined as enzymes that catalyze the hydrolysis of ester bonds in substrates such as vitamin esters, phospholipids, triglycerides (TGs) and cholesteryl esters [1]. Interest in lipases has been increasing in the past few years because of the actual and potential uses of this enzyme in applications like hydrolysis of fats and oils, organic synthesis, modifica...

A lipolytic screening with fungal strains isolated from lignocellulosic waste collected in banana plantation dumps was carried out. A Trichoderma harzianum strain (B13-1) showed good extracellular lipolytic activity (205 UmL-1). Subsequently, functional screening of the lipolytic activity on Rhodamine B enriched with olive oil as the only carbon source was performed. The successful growth of th...

BACKGROUND Lipases are regularly used in biotechnology to catalyse the hydrolysis of triglycerides and the synthesis of esters. Microbial lipases in particular have been widely used in a variety of industrial applications. However, the current commercial microbial lipases cannot meet industrial demand due to rapid inactivation under harsh conditions. Therefore, in order to identify more stable ...

Lipase engineering database http://www.led.uni-stuttgart.de/ Lipases are heavily used in industry, especially for chiral resolution to produce compounds enantiospecifically. This database deals specifically with lipases. Directory of P450-containing systems http://www.icgeb.org/~p450srv/ Cytochrome P450 enzymes are widespread in biological systems, including bacteria and fungi. These monooxygen...

Currently bacterial lipases are of great demand because of potential industrial applications (Sirisha et al., 2010). Lipases find their applications in various industrial sectors like processing of fats and oils, detergents and degreasing formulations, food processing, chemical and pharmaceuticals, paper mills, etc (Rubin and Dennis, 1997a,b; Kazlauskas and Bornscheuer, 1998; Masse et al., 2001...

This review focuses on the kinetics and mechanisms of reactions catalysed by immobilized lipases. The effects of pH, temperature, and various substances on the catalytic properties of immobilized lipases and on the processes by which they are deactivated are reviewed and discussed.

Dual promiscuous racemization/amidation activities of lipases leading to efficient dynamic kinetic resolution protocols of racemic α-aminonitrile compounds are described. α-Amidonitrile products of high enantiomeric purity could be formed in high yields. Several lipases from different sources were shown to exhibit the dual catalytic activities, where opposite enantioselectivities could be recor...

Upgrading of farmed salmon oil obtained from by-products was carried out by lipase-catalyzed hydrolysis to increase omega 3 polyunsaturated fatty acids (PUFA) content. The lipases tested were from Penicillium camembertii (PC), Rhizomucor javanicus (RJ), Rhizopus niveus (RN), Rhizopus delemar (RD), Burkholderia cepacia (BC), Rhizopus oryzae (RO), Candida rugosa (CR) and Rhizomucor miehei (RM). T...

Three kinds of lipases (from Candida antarctica, Pseudomonas cepacia, and Pseudomonas fluorescens) were encapsulated in inorganic matrices by the sol-gel method in order to synthesize chiral compounds by kinetic resolution. Sol-gel lipases prepared with vinyltriethoxysilane had higher hydrolysis activity for 2-octyl acetate than those with other silane precursors: tetramethoxysilane, methyltrim...