We have developed a gene transfection method called water-in-oil droplet electroporation (EP) that uses a dielectric oil and a liquid droplet containing live cells and exogenous DNA. When a cell suspension droplet is placed between a pair of electrodes, an intense DC electric field can induce droplet deformation, resulting in an instantaneous short circuit caused by the droplet elongating and c...

The ionic activities and total molalities of sodium, potassium, calcium, lithium, and chloride in a solution of human serum albumin were measured at different values of pH between 4 and 9. The same quantities were measured simultaneously in a protein-free electrolyte solution in membrane equilibrium with the albumin solution. Taking the residual liquid-junction potential and bias from unselecti...

a physic-mathematical model has been developed that is suitable for biosensors incorporating membranes and bisubstrate enzymes. the set of equations regarding the transport of reactants across the membrane, the bisubstrate enzymatic reaction, back diffusion of reactants and reaction products, are written down and simultaneously solved and transient response of the electrode is determined as a f...

in this project, eggshell membrane was used as a solid phase extraction sorbent for extraction of morphine followed by hplc analysis. at first, raw eggshell membrane was used and then, because of low adsorption of morphine on untreated eggshell membrane (

the present paper concerns on the selective and very efficient transport of thorium(iv) ions from aqueous solutions through an emulsion liquid membranes composed by paraffin and a surfactant, without carrier requirement. the influence of ph of the external aqueous phase, the surfactant concentration in the membrane phase, the type, and concentration of the acid used in the receiving phase, the ...

this paper focuses on the effects of the addition of an ionic liquid, 1-allyl-3-butylimidazilium bis(trifluoromethane sulfonyl)imide ([abim]tfsi), which has a high affinity for benzene, into the poly(vinyl chloride) (pvc) membrane on the pervaporation characteristics of the removal of benzene from aqueous solutions of dilute benzene. when aqueous solutions of 100～500 ppm benzene were permeated ...

traditional systems for olefin/paraffin separation, like low-temperature distillation, are expensive and very energy consuming, and therefore, alternative separation methods are desired. facilitated transport membranes are new tools for this separation to be substituted in large scale separation of olefin-paraffin mixtures. to design a membrane process for olefin-paraffin separation, equilibriu...

The recent boom of energy storage and conversion devices, exploiting ionic liquids (ILs) to enhance the performance, requires an in-depth understanding of this new class of electrolytes in device operation conditions. One central question critical to device performance is how the mobile ions accumulate near charged electrodes. Here, we present the excess ion depth profiles of ILs in ionomer mem...

A fabrication scheme to realize dense arrays of flexible, closed membranes with a small gap separating them from the substrate is presented. These membranes are the first step towards aquatic hair based flow sensors biomimicking fish lateral line. Electrodes are integrated underneath the membrane to avoid contact with the liquid. Arrays of membranes with a diameter of 100 μm, gap height of 3 μm...

The performance of an optimized tree-type cylindrical-shaped nanoporous fltering membrane proposed in the former study is analytically investigated for varying operational parameter values. Across the membrane thickness, this membrane has two-leveled tree-structured pores including one trunk pore and the multiple branch pores. The physical properties of the surfaces of ...