Non-Alcoholic Fatty Liver Disease (NAFLD) is one of the most important causes liver disease worldwide leading foreground cause transplantation. Recently miRNAs, small non-coding molecules were identified as an player in negative translational regulation many protein-coding genes involved hepatic metabolism. Visceral adipose tissue was found to take part lipid and glucose metabolism release infl...

results the allele and genotype distributions of pnpla3 rs738409 and rs2281135 were not significantly different between the chb and lc groups. after segregation on the basis of sex, no significant correlation between pnpla3 (rs738409 and rs2281135) genotypes/alleles and liver cirrhosis was detected. moreover, none of the haplotypes in pnpla3 (rs738409 and rs2281135) was found to be statisticall...

Free cholesterol has been considered to be a critical risk factor of nonalcoholic fatty liver disease (NAFLD). It remains unknown whether dietary intake condensed tannins (CTs) have distinguishable effects alleviate damage caused by high diet. Male C57BL/6 mice were fed diet for 6 weeks, and given CTs treatment at dosage 200 mg/(kg·bw·day) the same time. The results indicated that compared with...

The liver is the main organ responsible for drug metabolism; hepatocyte-based culture models play a fundamental role in understanding physiology. While different types of two-dimensional and three-dimensional have been developed, failure to accurately mimic native liver, including extracellular matrix (ECM) composition, complex structure, rich vascular network as well shortage donors, impede th...

Abstract Gene expression plays a pivotal role in the long-term regulation of gluconeogenesis and lipogenesis liver. Although most previous studies were analyzed as whole liver, it is known that hepatocytes across liver display specific aspects function zonation dependent manner. Using combination single-cell RNA sequencing (scRNA-seq) single molecule Fluorescence In Situ Hybridization (smFISH) ...

Liver sexual gene dimorphism, which depends mainly on specific patterns of GH secretion, may underlie differential susceptibility to some liver diseases. Because GH and prolactin secretion are regulated by dopaminergic pathways, we studied the participation of brain and lactotrope dopamine 2 receptors (D2Rs) on liver gene sexual dimorphism, to explore a link between the brain and liver gene exp...

This study aimed to investigate whether Opuntia spp-extracts protect against diclofenac (DF)-induced hepatotoxicity. Rats were pretreated with extracts, betanin (Bet) and N-acetylcysteine (NAC) followed by a single challenge of diclofenac. Liver tissue was collected for biochemical histological analysis. Primary rat hepatocytes treated (400 µmol/L) without pretreatment extract. Apoptosis measur...

Non-alcoholic fatty liver disease (NAFLD) represents a growing global health concern that can lead to and cancer. It is characterized by an excessive accumulation of fat in the liver, unrelated alcohol consumption. Studies indicate gut microbiota-host crosstalk may play causal role NAFLD pathogenesis, with epigenetic modification serving as key mechanism for regulating this interaction. In revi...

In this study, we screened the expression stability of six reference genes (18S rRNA, β-actin, GAPDH, EF1a, B2M, and HPRT1) in hybrid yellow catfish (n = 6), considering SBM levels, sampling time points, different tissues. Four statistical programs, BestKeeper, NormFinder, Genorm, Delta Ct, combined with a method that comprehensively considered all results, were used to evaluate these systemati...

A growing number of long noncoding RNAs (lncRNAs) have emerged as vital metabolic regulators. However, most human lncRNAs are nonconserved and highly tissue specific, vastly limiting our ability to identify lncRNA regulators (hLMRs). In this study, we established a pipeline putative hLMRs that metabolically sensitive, disease relevant, population applicable. We first progressively processed mul...